Single Column
InquireCatalog Number｜Packaging
Mat. No |
Ref. No |
No. of preps |
|
4992201 |
GDP305-02 | 50 |
|
4992202 |
GDP305-03 | 200 |
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Features
● Simple and fast: The purifed gDNA could be obtained within 1 hour.
● High purity and excellent quality: The highly pure genomic DNA could be used directly in downstream experiments such as PCR amplifcation, restriction endonuclease digestion and Southern blot.
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Description
The Plant Genomic DNA Kit provides a fast, simple, and cost-effective genomic DNA extraction method for routine molecular biology laboratory applications. It’s especially suitable for genomic DNA purification from powder or polysaccaride/polyphenol rich plants. The kit is based on the silica membrane technology and a unique bufer to eliminate polysaccaride, polyphenol, and enzyme inhibitors. Plant Genomic DNA Kit is ready-to-use to purify the genomic DNA from a wide variety of plants. The purifed DNA is suitable for PCR, restriction digestion and Southern blot. -
Kit Contents
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Applications
● Suitable for various plant tissues.
● Particularly suitable for polysaccaride/polyphenol-rich plant tissues.
● Particularly suitable for plant powders.
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Required Reagents
Liquid nitrogen, Ethanol, Phenol-chloroform, RNaseA (optional)
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Yield For Reference
Note: DNA yield depends on sample types. All the materials above are from tender leaves.
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Storage Condition
Plant Genomic DNA Kit could be stored dry at room temperature (15-30°C) for up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C for 10 min before use. -
Important Notes
● Repeated freezing and thawing should be avoided; otherwise it would reduce the DNA size and quantity.
● If a precipitate formed in Buffer GP1 or Buffer GP2, warm buffer to 37°C until the precipitate has fully dissolved.
Experimental Example
Genomic DNA isolated from 100 mg leaves of different plants using Plant Genomic DNA Kit. 3 μl of 100 μl eluates were loaded per lane.
1: Fresh tomato leaves;
2: Fresh cotton leaves;
3: Fresh tea leaves;
4: Fresh strawberry leaves.
The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.
M: λDNA/Hind III Marker
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