Single Column
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Features
● Short operation time: High quality yeast gDNA could be obtained within one hour.
● Non-toxic: The operation is safe since no phenol or chloroform extraction is needed.
● High purity: Pure DNA can be obtained by using this kit, which can be directly used in downstream molecular biology experiments such as PCR, restriction digestion, Southern blotting, etc.
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Description
The TIANamp Yeast DNA Kit adopts a highly efficient DNA-binding spin column and a unique buffer system to extract yeast genomic DNA. After the sample is lysed, the DNA specifically binds to the silica membrane under high salt condition. The DNA then elutes from the silica membrane under low salt and high pH condition. The DNA purified by this kit can be applied in various tests, including restriction digestion, PCR and Southern blot. -
Kit Contents
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Applications
● PCR and qPCR.
● Restriction digestion, library construction, Southern blotting.
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Required Reagents
Sorbitol buffer, lyticase, ethanol, RNase A (optional). -
Storage Condition
Room temperature (15-30℃). -
Important Notes
● Repeated freezing and thawing of stored samples should be avoided, since this leads to reduced DNA size.
● If a precipitate has formed in Buffer GA or Buffer GB, warm buffer to 37°C until the precipitate has fully dissolved.
● All centrifugation steps should be carried out in a conventional table-top microcentrifuge at room temperature (15-30°C).
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