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Storage
Room temperature (15-25℃) -
Description
The TIANamp Yeast DNA Kit adopts a highly efficient DNA-binding spin column and a unique buffer system to extract yeast genomic DNA. After the sample is lysed, the DNA specifically binds to the silica membrane under high salt condition. The DNA then elutes from the silica membrane under low salt and high pH condition. The DNA purified by this kit can be applied in various tests, including restriction digestion, PCR and Southern blot. -
Required Reagents
Sorbitol buffer, lyticase, ethanol, RNase A (optional) -
Features
■ Short operation time: High quality yeast gDNA could be obtained within one hour.
■ Non-toxic: The operation is safe since no phenol or chloroform extraction is needed.
■ High purity: Pure DNA can be obtained by using this kit, which can be directly used in downstream molecular biology experiments such as PCR, restriction digestion, Southern blotting, etc.
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Applications
■ PCR and qPCR.
■ Restriction digestion, library construction, Southern blotting
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