Precipitation Method

DNAquick Plant System

Rapid purification of high quality genomic DNA from various plant tissues with different starting amount according to specific experimental requirements

Catalog number / packaging

Cat. no

ID

No. of preps

4992710

DP321-03 200

4992709

DP321-02 50
  • Storage

    Room temperature (15-25℃)
  • Description

    The DNAquick Plant System adopts a unique buffer system to extract genomic DNA from a variety of fresh or cryopreserved plant tissues. This kit does not require phenol or chloroform extraction and is safe to operate. It can extract large fragments of genomic DNA with high purity and stable quality.
  • Required Reagents

    Liquid nitrogen, isopropanol, 70% ethanol
  • Features

    ■ Simple and fast: The kit is provided as liquid system, and high quality purified gDNA could be obtained within 1 hour from various plant tissues rapidly.

    ■ Non-toxic: The operation is safe since no phenol or chloroform extraction is needed.

    ■ No limit for the starting amount of the materials: Users can adjust the initial amount of the samples according to specific experimental requirements flexibly.

    ■ High cost-effective: The good quality and popular price makes the kit very suitable for large amount plant genome extraction.

  • Applications

    ■ PCR, qPCR and mutiplex PCR.

    ■ DNA profiling techniques such as RAPD, RFLP, AFLP, SSR, etc.

    ■ Library construction, Southern blot.

  • DNA Extraction Yield From Various Plant Tissues:

Experimental Example

  • Genomic DNA isolated from 100 mg leaves of different plants using DNAquick Plant System. 3 μl of 100 μl eluates were loaded per lane.

    1: Fresh soybean leaves;

    2: Fresh tobacco leaves;

    3: Fresh wheat leaves;

    4: Fresh corn leaves.

    The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.

    M: λDNA/Hind III Marker