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TIANquick FFPE DNA Kit

One-hour quick purification of DNA from formalin-fixed, paraffin-embedded tissues without xylene treatment

Catalog number / packaging

Cat. no

ID

No. of preps

4992296

 DP330-02 50
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃)

  • Description

    The TIANquick FFPE DNA Kit provides special lysis condition and unique deparaffinization method to extract DNA from formalin-fixed and paraffin-embedding (FFPE) samples. This kit significantly reverses the cross-linking and minimizes the impairment of DNA caused by FFPE procedure. It also incorporates specific adsorption column which completely avoid using of toxic and hazardous organic reagents such as xylene and the overnight digestion with proteinase K. The purified DNA is suitable for diagnosis and academic research.

  • Required Reagents

    Ethanol, RNaseA (optional)

  • Features

    ■ The whole procedure could be finished within one hour.

    ■ Neither xylene nor other hazardous solvents is needed.

    ■ High-purity DNA could be isolated without proteinase K overnight digestion.

  • Applications

    ■ PCR and RT-qPCR

    ■ SNP and STR gene analysis

    ■ Pharmacogenomics research

Experimental Example

  • The genomic DNA extracted by TIANquick FFPE DNA Kit and relevant product from Supplier A were amplified to 190 bp, 300 bp, 750 bp fragments. The result shows that the PCR amplification efficiencies of the gDNA extracted by TIANquick FFPE DNA Kit and Supplier A were equivalent

    Starting material:  Mouse liver FFPE samples (5 pieces, 10 μm/piece)

    Procedure:  Follow the instructions of each product.

    Gel loading: gDNA was eluted by 50 μl elution buffer and was amplified to 190 bp, 300 bp and 750 bp. 5μl of 20 μl PCR products were loaded per lane on 1% agarose gel. The electrophoresis was conducted at 6 V/cm for 20 min.

    M: TIANGEN Marker D2000;

    T: TIANquick FFPE DNA Kit;

    A: Similar product from Supplier A.

Publication

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