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Single Column

TIANamp FFPE DNA Kit

High-efficient purification of DNA from formalin-fixed, paraffin-embedded tissues by xylene treatment

Catalog number / packaging

Cat. no

ID

No. of preps

4992524

 DP331-02 50
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃)

  • Description

    The TIANamp FFPE DNA Kit applies xylene deparaffinization method to remove paraffin, and provides special lysis condition to extract DNA from formalin-fixed and paraffin-embedding (FFPE) samples. This method overcomes the inhibitory effect of formalin cross-linking. By combining the specific columns and a special buffer system, high-quality DNA can be extracted into a small elution volume. The purified DNA has good genome integrity, high purity and stable and reliable quality, which is ideally suitable for diagnosis and academic research.

  • Required Reagents

    Xylene, ethanol, RNaseA (optional)

  • Features

    ■ Wide application: The kit could be used to extract gDNA from various FFPE samples.

    ■ Easy extraction procedure:  Easy extraction and purification of high-quality, high-yield and ready-to-use DNA with good repeatability.

    ■ Stable and reliable: Complete removal of contaminants and PCR inhibitors.

  • Applications

    ■ PCR and RT-qPCR

    ■ SNP and STR gene analysis

    ■ Pharmacogenomics research

Experimental Example

  • The genomic DNA extracted by TIANamp FFPE DNA Kit and relevant product from Supplier A were amplified to 190 bp, 330 bp, 500 bp, 650 bp and 1300 bp fragments. The result shows that DNA extracted by TIANamp FFPE DNA Kit has higher yield, higher purity especially for large fragments.

    Starting material: Mouse liver FFPE samples (5 pieces, 10 μm/piece)

    Procedure: Follow the instructions of each product.

    Gel loading: gDNA was eluted by 50 μl elution buffer and was amplified to 190 bp, 330 bp 500 bp, 650 bp and 1300 bp. 5 μl of 20 μl PCR products were loaded per lane on 1% agarose gel. The electrophoresis was conducted at 6 V/cm for 20 min.

    M: TIANGEN Marker D2000;

    T: TIANamp FFPE DNA Kit;    

    A: Relevant product from Supplier A.

Publication

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