Single Column
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Features
● Wide application: The kit could be used to extract gDNA from various FFPE samples.
● Easy extraction procedure: Easy extraction and purification of high-quality, high-yield and ready-to-use DNA with good repeatability.
● Stable and reliable: Complete removal of contaminants and PCR inhibitors.
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Description
The TIANamp FFPE DNA Kit applies xylene deparaffinization method to remove paraffin, and provides special lysis conditions to extract DNA from formalin-fixed and paraffin-embedding (FFPE) samples. This method overcomes the inhibitory effect of formalin cross-linking. By combining the specific adsorption columns and a special buffer system, high-quality DNA can be extracted into a small elution volume. The purified DNA has good genome integrality, high purity and stable and reliable quality, which is ideally suitable for diagnosis and academic research.
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Kit Contents
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Applications
● PCR and RT-qPCR
● SNP and STR gene analysis
● Pharmacogenomics research
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Required Reagents
Xylene, ethanol, RNase A (optional). -
Storage Condition
Room temperature (15-30℃). -
Important Notes
● Fix tissue samples in 4-10% formalin as quickly as possible after surgical removal. Use a fixation time of 8-24 hours (longer fixation time lead to more severe DNA fragmentation, resulting in poor performance in downstream assays).
● Thoroughly dehydrate samples prior to embedding (residual formalin can inhibit PCR reaction).
● This kit applies in medical and scientific research.
● The integrity of DNA obtained in this kit depends on the samples type, storage and fixation conditions. Longer fixation time or longer storage time (>1 year) will lead to DNA fragmentation, in this case, long fragments will not be amplified.
● If a precipitate has formed in Buffer GA or Buffer GB or Buffer GD, warm it at 37°C until the precipitate has fully dissolved.
● Each reagent in the kit should be performed according to this instruction.
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Experimental Example
The genomic DNA extracted by TIANamp FFPE DNA Kit and relevant product from Supplier A were amplified to 190 bp, 330 bp, 500 bp, 650 bp and 1300 bp fragments. The result shows that DNA extracted by TIANamp FFPE DNA Kit has higher yield, higher purity especially for large fragments.
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