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Single Column

Serum / Plasma Circulating DNA Kit

For isolation of genomic DNA from plasma and serum

Catalog number / packaging

Cat. no

ID

No. of preps

4992289

 DP339 50
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃)

  • Description

    The Serum/Plasma Circulating DNA Kit is based on silica membrane technology and provides special buffer system. Genomic DNA binds to the silica-membrane in the presence of high salt, while the contaminants pass through the column. After the membrane is thoroughly washed to remove all remaining contaminants, the pure DNA is eluted from the membrane with low salt buffer. The purified DNA can be directly used in downstream molecular biology experiments such as PCR and real time qPCR, etc.


  • Required Reagents

    Ethanol, RNaseA (optional)

  • Features

    ■ The kit can extract circulating DNA simply and fast by adopting the silica membrane spin column.

    ■ Safe and non-toxic because there’s no need for phenol/chloroform extraction.

    ■ Carrier RNA enhances binding of low abundance DNA to spin column membrane.

    ■ Complete removal of contaminants and PCR inhibitors for downstream experiments.

  • Applications

    PCR and real-time qPCR

Experimental Data

  • DNA purified from 400 μl plasma samples using Serum/Plasma Circulating DNA Kit. The following are results of Agilent 2100 analysis after library construction.

    1. The circulating DNA purified by Serum/Plasma Circulating DNA Kit is of high purity, and no nonspecific fragments have been detected.

    2. The circulating DNA has high recovery rate.

  • DNA purified from 400 μl plasma samples using Serum/Plasma Circulating DNA Kit. The following are results of Agilent 2100 analysis after library construction.

    Group A: Caliper analysis of the circulating DNA extracted from 200 μl plasma by Serum/Plasma Circulating DNA Kit.

    Group B: Caliper analysis of the circulating DNA extracted from 600 μl plasma by a relevant product from another supplier.

    1. The circulating DNA purified by Serum/Plasma Circulating DNA Kit is of higher purity, and fewer nonspecific fragments have been detected.

    2. The recovery rate of circulating DNA is higher using Serum/Plasma Circulating DNA Kit. The DNA yield from 200 μl plasma is equivalent to the yield from 600 μl plasma by other kits, suggesting that Serum/Plasma Circulating DNA Kit is more cost-effective.

Publication

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