-
Features
● Simple and fast: Ultra-pure gDNA could be obtained within 1 hour.
● Wide applications: Suitable for various plant tissues, especially for polysaccharides & polyphenolics-rich plants.
● Non-toxic: Safe operation because no need of phenol or chloroform for extraction.
● High purity and efficiency: Ultra-pure DNA can be obtained efficiently, which can be applied directly in molecular biology experiments such as chip hybridization, NGS, etc.
-
Description
The Super Plant Genomic DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system, which can separate and purify high-quality genomic DNA from a variety of plant tissues. The unique precipitation solution can precipitate and remove the protein, polysaccharide, phenol and other impurities in the polysaccharides & polyphenolics-rich plant samples. The extracted genomic DNA has high purity, stability and reliable quality.
-
Kit Contents
-
Required Reagents
96-100% ethanol -
Storage Condition
This kit can be stored dry for 15 months under room temperature (15-30°C). For long-term storage, store at 2-8°C.
When stored at 2-8°C, precipitation might be formed in buffer. Place the buffers at room temperature for a period of time before use, and if necessary, incubate the buffers in a 37°C water bath for 10 minutes to dissolve the precipitation.
-
Important Notes
● Avoid repeated freezing and thawing, otherwise the DNA fragments extracted will be smaller and the amount of extraction will be reduced.
● If there's precipitation in Buffer GPS, please dissolve it at 37°C and shake it before use.
-
Experimental Example
-
Measurement of DNA Concentration and Purity
● The size of the genomic DNA fragment obtained is related to the sample preservation time, the shearing force during the operation and other factors. The concentration and purity of recovered DNA fragments can be detected by agarose gel electrophoresis and ultraviolet spectrophotometer.
● DNA should have a significant absorption peak at OD260. If the OD260 value is 1, then it is equivalent to about 50 μg/ml double-strand DNA and 40 μg/ml single-strand DNA.
● The OD260/OD280 ratio should be 1.7-1.9. If it is not the elution buffer but deionized water is used, the ratio will be low, because the pH value and the presence of ions will affect the light absorption value, but it does not mean the purity is low.
-
Sort by
-
Date
Date(
)
Date
Date(
)
Impact Factor
IF(
)
Impact Factor
IF(
)