Magnetic Beads-based Method

Magnetic Blood Genomic DNA Kit

Highly efficient purification of high quality genomic DNA from 100 μl-1 ml blood

Catalog number / packaging

Cat. no

ID

No. of preps

4992976

DP329-03 1000

4992403

DP329-02 200

4992402

DP329-01 50
  • Storage Conditions

    Store at room temperature (15-25℃ ).
  • Description

    The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality genomic DNA from 100 μl-1 ml of blood. Unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions. When the conditions change, the magnetic beads release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid. The whole process is safe and convenient. With large extracted genomic DNA fragments, high purity and reliable quality, it is especially suitable for automatic extraction of high-throughput workstations. The DNA purified by the kit can be suitable for various conventional operations, including enzyme digestion, PCR, real time PCR, library construction, Southern blot, chip detection, high throughput sequencing and other experiments.

  • Features

    ■ Simple and fast: Ultra-pure genomic DNA can be obtained within 1 hour.

    ■ High Throughput: It can integrate the automated instrument of pipetting and magnetic rod to carry out high throughput extraction experiments.

    ■ Safe and non-toxic: No toxic organic reagents such as phenol/ chloroform are needed.

    ■ High purity: The obtained DNA has high purity and can be directly used in chip detection, high-throughput sequencing and other experiments.

  • Required Instruments

    Magnetic stand, or automated pipetting instruments such as Hamilton Microlab STAR, Beckman Coulter Biomek FX and Capitalbio LabKeeper, or automated magnetic rod instruments such as Thermo KingFisher Flex and Taco.
  • Required Reagents

    Isopropanol, ethanol
  • Extraction Yield

Experimental Data

  • DNA NanoDrop measurement results of DNA extracted from 200 μl blood using TIANGEN Magnetic Blood Genomic DNA Kit and relevant product from Supplier G

  • Use TIANGEN Magnetic Blood Genomic DNA Kit and relevant product from Supplier G to extract DNA from 200 μl of blood. DNA was eluted with 200 μl Buffer TB. Take 5 μl for electrophoresis, and 2 μl for NanoDrop 2000 to measure concentration and purity. The results show that the extraction yield of TIANGEN Magnetic Blood Genomic DNA Kit is 18% higher than that of Supplier G.

    M: TIANGEN Marker D15000