Single Column
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Features
● Simple and fast: Ultra-pure genomic DNA can be obtained within 1 h.
● Ultra-pure: The extracted gDNA is of high purity, which can be directly used in molecular biology experiments such as PCR, restriction digestion and Southern blot.
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Description
The TIANamp Blood Clot DNA Kit adopts the spin column that specifically binds DNA and a unique buffer system to extract genomic DNA from blood clots. The silicon matrix material used in the spin column is a unique new material developed by TIANGEN, which can efficiently and specifically adsorb DNA, and remove impurities and other organic compounds in cells to the utmost extent. Large size of gDNA fragments with high purity and stability can be obtained using this kit. -
Kit Contents
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Applications
Suitable for various routine applications, such as restriction digestion, PCR, library construction, Southern blot, etc. -
Required Reagents
Ethanol, RNase A (optional) -
Storage Condition
TIANamp Blood Clot DNA Kit can be stored dry at room temperature (15- 30°C) for up to 15 months without showing any reduction on performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C before use. -
Important Notes
● Ethanol (96-100%) should be added to Buffer GD and Buffer PW as indicated on the bot before use.
● Samples should not be frozen and thawed repeatedly, or else the integrity and yield of DNA would be affected negatively.
● If precipitates have been formed in Buffer GB, dissolve them by incubating at 37°C.
● All centrifugation steps should be carried out in a conventional table-top microcentrifuge at room temperature (15-30°C).
● If the blood clot sample is more than 1 ml, please purchase TIANGEN TIANamp Blood DNA Midi Kit (4992854) and Liquefaction Columns CX2/CX3.
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Experimental Example
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