Menu
English

English

Janpanese(Japan)

Korean(Korea)

Precipitation Method

RelaxGene Blood DNA System(0.1-20ml)

Extraction of genomic DNA from 0.1-20 ml fresh and cryopreserved blood of various anticoagulants

Catalog number / packaging

Cat. no

ID

No. of preps

4992722

 DP349-01 50 ml

4992723

 DP349-02 200 ml
Manual Inquire Inspection report

  • Storage

    This kit can be stored under dry condition at room temperature (15-25℃ ) for 12 months. For longterm storage, please store at 2-8℃ .

  • Description

    The RelaxGene Blood DNA System adopts a unique buffer system to extract genomic DNA from 0.1-20 ml of fresh blood with various anticoagulants and cryopreserved blood samples. The buffer system can remove the proteins, pigments, lipids and other inhibitory impurities to a maximum extent, and obtain large size DNA fragments with high yield, high purity and good stability. The kit does not need to use organic solvents such as phenol/chloroform. The purified DNA can be used in various regular experiments such as restriction digestion, PCR, library construction, Southern blot (high throughput sequencing chip hybridization), etc. 

  • Features

    ■ Flexible sample starting amount: 2-400 μg high purity DNA can be extracted from 0.1-20 ml of various blood samples.

    ■ Safe and reliable: No contamination of organic solvents such as phenol/chloroform.

  • Applications

    Suitable for various regular applications, such as restriction digestion, PCR, library construction, Southern blot (chip hybridization of high-throughput sequencing), etc.

  • Purification of the genomic DNA from 300 μl, 5 ml and 10 ml human anticoagulation blood with EDTA using RelaxGene Blood DNA System . 3 μl of the 200 μl, 500 μl and 1 ml eluates respectively were loaded per lane. M: TIANGEN Marker D15000.

    Experimental results: 0.1-20 ml of blood can be extracted by RelaxGene Blood DNA System with good linear range.

  • Comparison of the genomic DNA extracted from 300 μl human anticoagulation with EDTA using RelaxGene Blood DNA System and similar products from other suppliers. M: TIANGEN Marker D15000. 3 μl of 200 μl eluates were loaded per lane.

    Experimental results: Compare to other suppliers, RelaxGene Blood DNA System can obtain higher yield, purity and integrality of blood gDNA.

  • Genomic DNA were extracted from blood samples using RelaxGene Blood DNA System.

    Samples: 300 μl human anticoagulation blood with EDTA, EDTA/NaF, heparin and sodium citrate; buffy coat from 2 ml blood; 10 μl chicken blood and 200 μl rat blood. 3 μl of 200 μl eluates were loaded per lane. M:TIANGEN Marker D15000.

    Experimental results: RelaxGene Blood DNA System can be widely applied in the high quality DNA purification of human anticoagulants with different treatments as well as the blood from various species.

  • Other Reference Data

Publication

Inquire