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TIANamp Feedstuff Animal DNA Kit

Purification of high quality genomic DNA from various feedstuff

Catalog number / packaging

Cat. no

ID

No. of preps

4992711

 DP323-02 50

4992712

 DP323-03 200
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃ )

  • Description

    TIANamp Feedstuff Animal DNA Kit is specifically developed for the safety detection of animal sources such as cattle and sheep in cooperation with the Feed Quarantine Department of the Ministry of Agriculture. This kit can extract genomic DNA from a variety of animal derived plant feedstuff or animal tissues. It can extract large fragments of genomic DNA with high purity and stable quality. The purified gDNA can be applied to various regular applications, including PCR and real-time qPCR for the feedstuff safety detection.

  • Required Reagents

    Ethanol, RNaseA (optional)

  • Features

    ■ Highly targeted: Specifically developed in cooperation with the Feed Quarantine Department.

    ■ Wide applications: Applicable for a variety of feedstuff and animal tissues.

    ■ High yield and detection sensitivity: 500 ng-40 μg genomic DNA can be extracted from 50 mg feedstuffs with the detection sensitivity of 0.1%.

  • Applications

    ■ PCR detection

    ■ qPCR detection

  • Feedstuff genomic DNA extraction by TIANamp Feedstuff Animal DNA Kit 

    Starting amount: 50 mg. 3 μl of 100 μl eluates were loaded per lane.

    1: Blank feedstuff;

    2: Cattle source positive feedstuff 1;

    3: Cattle source positive feedstuff 2;

    4: Sheep source positive feedstuff 1;

    5: Sheep source positive feedstuff 2.

    The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.

  • PCR detection of feedstuff gDNA by specific primers of cattle and sheep.

    1: Blank specific primers of sheep;

    2: Blank specific primers of cattle;

    3: Specific primers of cattle source positive feedstuff 1;

    4: Specific primers of cattle source positive feedstuff 2;

    5: Specific primers of sheep source positive feedstuff 1;

    6: Specific primers of sheep source positive feedstuff 2.

    The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.

    M: TIANGEN D2000

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