Magnetic Beads-based Method

Magnetic Blood Spots DNA Kit

Magnetic bead method kit capable of purifying DNA in high-throughput rapid from dry blood spot

Catalog number / packaging

Cat. no

ID

No. of preps

4992720

DP344-01 50

4992721

DP344-02 200

4992914

DP344-03 1000
  • Storage

    Under the condition of drying at room temperature (15-25℃ ), it can be stored for 12 months and stored at 2-8℃ for a longer time. 

  • Description

    The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality genomic DNA from dried blood spots. Unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions. When the conditions change, the magnetic beads release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid. The whole process is safe and convenient. With large extracted genomic DNA fragments, high purity and reliable quality, the method is especially suitable for automatic extraction with high-throughput workstations.

  • Features

    ■ Simple and fast: Ultra-pure genomic DNA can be obtained within 1 hour.

    ■ High Throughput: This kit can be integrated with the automated instruments of pipetting method and magnetic rod method to carry out high throughput extraction experiments.

    ■ Safe and non-toxic: No organic reagents such as phenol/chloroform is needed.

    ■ High purity: The obtained DNA has high purity and can be directly used in chip detection, high-throughput sequencing and other experiments.

  • Applications

    The DNA recovered by the kit can be suitable for various regular operations, including enzyme digestion, PCR, library construction, Southern blot and other experiments. 

  • DNA was extracted from three pieces of 3 mm diameter dry blood spot slides by spin column method TIANamp Blood Spots DNA Kit (4992716) and Magnetic Blood Spots DNA Kit (4992720), respectively, and detected by real time PCR using SuperReal PreMix Plus (4992214). The results show that the Ct value of DNA sample extracted by Magnetic Blood Spots DNA Kit is lower than that of spin column method, which proves that the extraction rate of Magnetic Blood Spots DNA Kit is higher than that of spin column method.

  • DNA was extracted from different numbers of dry blood spot slides by TIANGEN Magnetic Blood Spots DNA Kit. M:TIANGEN Marker D15000.The results show that there is a good linear relationship between dry blood spots with different number of slices.

  • DNA was extracted from six pieces of dry blood spot slides with TIANGEN Magnetic Blood Spots DNA Kit and relevant products from Supplier CW, O and M respectively. DNA was eluted with 200 μl Buffer TB, and 5 μl was loaded for electrophoresis. The results show that the extraction rate of Magnetic Blood Spots DNA Kit is higher than that of other suppliers. M: TIANGEN Marker D15000.