● Simple and fast: Ultra-pure gDNA could be obtained within 1 hour.

● High throughput: This kit can be integrated with the automated instruments of pipetting method and magnetic rod method to carry out high throughput extraction experiments.

● Wide applications: Can be widely used to extract DNA from tissues, bacteria, blood, saliva, swabs, etc.

● High purity: The obtained DNA has high purity and can be directly used in chip detection, high-throughput sequencing and other experiments.

The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality genomic DNA from all kinds of blood, dry blood spots, saliva, swabs, FFPE, bacteria, animal tissues and other samples. Unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions. When the conditions change, the magnetic beads release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid. The whole process is safe and convenient. With large extracted genomic DNA fragments, high purity and reliable quality, the method is especially suitable for automatic extraction with high-throughput workstations.

Suitable for a variety of routine operations, including restriction enzyme digestion, PCR, real-time PCR, library construction, chip hybridization, Southern blot and high-throughput sequencing.

This kit can be stored at room temperature (15-30 °C) under dry condition for 15 months. If a precipitate has formed in Buffer, please place the buffer at 37 °C for 10 min to dissolve the precipitate.

● This product is suitable for manual extraction or automatic instrument integration.

● Avoid repeated freezing and thawing of the sample, otherwise the extracted DNA fragments will be smaller and the extraction yield will also decrease.

● If there is precipitation in Buffer GHL, it can be redissolved in 37 °C water bath. Please use after shaking.

● Self-provided reagents: Isopropanol, ethanol. For tissue samples, 1M DTT should be prepared. For bacteria samples, 1M NaOH should be prepared. If RNA residues need to be removed, RNase A(100 mg/ml) solution should be prepared. For FFPE samples, please purchase

environmentally friendly deparaffinization oil DPR. To extract large volumes of blood and blood clots, please purchase Buffer CLA and Buffer GHL separately.

The size of the obtained genomic DNA fragment is related to factors such as sample storage time and shearing force during operation. The purified DNA fragments can be detected by agarose gel electrophoresis and UV spectrophotometer for concentration and purity. 

DNA should have a significant absorption peak at OD₂₆₀, with OD₂₆₀ value of 1 equivalent to about 50 μg/ml double stranded DNA and 40 μg/ml single stranded DNA. 

The ratio of OD₂₆₀/OD₂₈₀ should be 1.7-1.9. If ddH2O is used instead of elution buffer, the ratio will be lower, because the pH value and the presence of ions will affect the light absorption value, but it does not mean the purity is low.