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Magnetic Beads-based Method

Magnetic Soil And Stool DNA Kit

Magnetic kit capable of realizing high-throughout and rapid extraction of genomic DNA from soil/stool/intestinal microorganism

Catalog number / packaging

Cat. no

ID

No. of preps

4992736

 DP712-01 50 preps

4992738

 DP712-02 200 preps
Manual Inquire Inspection report

  • Storage

    The kit can be stored for 12 months at room temperature (15-25°C) and 2-8°C for a longer time. Under the storage condition of 2-8°C, if precipitate appears, leave the solution in the kit at room temperature for a period of time before use, and if necessary, incubate in a 37°C water bath for 10 min to dissolve the precipitation.

  • Description

    The kit adopts a unique buffer system capable of removing humic acid from the soil sample as much as possible. It is provided with glass beads which can effectively crush various complex components in the soil sample and ensure the integrity of extracting genomic DNA from the soil. It is also suitable for the extraction of stool samples and intestinal microorganisms.

  • Required Reagents

    Isopropanol, ethanol.

  • Features

    ■ Wide applicability: Suitable for extraction of various types of soil environmental samples such as flower bed soil, flower pot soil, farmland soil, mountain forest soil, silt, red soil, black soil, dust and so on. It is also suitable for the extraction of stool samples and intestinal microorganisms.

    ■ Convenient operation: The experimental operation can be completed in a relatively short time.

    ■ High purity: Combined with magnetic bead purification, the extracted DNA has high purity and can be directly used in downstream experiments

  • Applications

    The DNA purified by the kit has few impurities and good integrity, and can be directly used in other downstream experiments of molecular biology such as PCR, enzyme digestion, etc.

  • Genomic DNA was extracted from 500 mg of garden soil by shaker and grinder respectively, using TIANGEN Magnetic Soil And Stool DNA Kit and relevant product from Supplier M. 5 μl of 100 μl eluate was loaded to 1% agarose gel electrophoresis. M: TIANGEN Maker D2000

  • Genomic DNA was extracted from human stools with TIANGEN Magnetic Soil And Stool DNA Kit and relevant product from Supplier M respectively, and was detected by PCR with 16S primers of bacteria. 5 μl of 20 μl PCR product was loaded to 1% agarose gel electrophoresis. M: TIANGEN Maker D2000

  • Genomic DNA was extracted from fish intestinal microorganisms using TIANGEN Magnetic Soil And Stool DNA Kit and relevant product from Supplier M respectively, and was detected by PCR with general primer 27F/1492R of bacterial with the product about 1500 bp. 5 μl of 20 μl PCR product was loaded to 1% agarose gel electrophoresis. M: TIANGEN Maker D2000

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