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Column-based Method

RNAprep Pure Tissue Kit

For purification of up to 100 μg total RNA from animal tissues

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992236

 Gdp431 50
Manual Inquire Inspection report

  • Storage

    DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.

    Other reagents could be stored at room temperature (15-25℃)

    Note: It is recommended to use RNAstore Reagent (4992727) to store the tissue samples.

  • Description

    The RNAprep Pure Tissue Kit provides a fast, simple, and cost-effective method for purification of total RNA from animal tissues by using effective spin column and unique buffer system. The kit includes RNase-Free spin columns CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 40-50 minutes with high-purity and is free of protein and genomic DNA contamination.

  • Required Reagents

    β-mercaptoethanol, ethanol

  • Features

    ■ Optimized buffers for animal tissues make the process simple and convenient.

    ■ Unique DNase I minimizes genomic DNA contamination.

    ■ The higher-purity and ready-to-use RNA is suitable for sensitive downstream applications.

    ■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, and no CsCl gradients centrifugation are needed, which makes the process safe and reliable.

  • Applications

    ■ RT-PCR.

    ■ Northern Blot, Dot Blot.

    ■ Real-Time PCR.

    ■ Chip analysis.

    ■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

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