Column-based Method
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RNAprep Pure Tissue Kit
For purification of up to 100 μg total RNA from animal tissues
Catalog number / packaging
Cat. no
ID
No. of preps
4992236
dp431 50
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Storage
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.
Other reagents could be stored at room temperature (15-25℃)
Note: It is recommended to use RNAstore Reagent (4992727) to store the tissue samples.
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Description
The RNAprep Pure Tissue Kit provides a fast, simple, and cost-effective method for purification of total RNA from animal tissues by using effective spin column and unique buffer system. The kit includes RNase-Free spin columns CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 40-50 minutes with high-purity and is free of protein and genomic DNA contamination. -
Required Reagents
β-mercaptoethanol, ethanol -
Features
■ Optimized buffers for animal tissues make the process simple and convenient.
■ Unique DNase I minimizes genomic DNA contamination.
■ The higher-purity and ready-to-use RNA is suitable for sensitive downstream applications.
■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, and no CsCl gradients centrifugation are needed, which makes the process safe and reliable.
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Applications
■ RT-PCR.
■ Northern Blot, Dot Blot.
■ Real-Time PCR.
■ Chip analysis.
■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.
