Menu
English

English

Janpanese(Japan)

Korean(Korea)

Column-based Method

RNAprep Pure Plant Kit

For purification of total RNA from plants and fungi

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992237

 Gdp432 50
Manual Inquire Inspection report

  • Storage

    DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.

    Other reagents could be stored at room temperature (15-25℃)

  • Description

    The RNAprep Pure Plant Kit provides a fast, simple, and cost-effective method for purification of total RNA from plant samples by using effective spin column and unique buffer system. The kit includes RNase-Free Filtration Column CS for homogenizing and filtering viscous plant or fungal lysates, and spin column CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes. The whole process is simple, easy and safe to operate with low toxicity. The obtained RNA has high purity and is free from protein contamination.

  • Required Reagents

    β-mercaptoethanol, ethanol

  • Features

    ■ Optimized buffers for plant samples make the process more convenient.

    ■ Unique DNase I minimizes genomic DNA contamination.

    ■ Unique filtration column CS eliminates other contaminations.

    ■ The high-purity ready-to-use RNA is suitable for sensitive downstream applications.

    ■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, and no CsCl gradients centrifugation are needed, which makes the process safe and reliable.

  • Applications

    ■ RT-PCR.

    ■ Northern Blot, Dot Blot.

    ■ Real-Time PCR.

    ■ Chip analysis.

    ■ PolyA Screening, in vitro translation, molecular cloning.

    Note:

    If sample is rich in secondary metabolism, Buffer HL provided by TIANGEN could be used to achieve maximum purification efficiency.

Sort by

Date Date()

Date Date()

Impact Factor IF()

Impact Factor IF()

Inquire