Column-based Method

RNAprep Pure Plant Kit

For purification of total RNA from plants and fungi

Catalog number / packaging

Cat. no	ID	No. of preps
4992237	dp432	50

Manual Inquire

Storage

DNase I, Buffer RDD, RNase-Free ddH₂O (Tubular) should be stored at 2-8℃.

Other reagents could be stored at room temperature (15-25℃)

DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.

Other reagents could be stored at room temperature (15-25℃)
Description

The RNAprep Pure Plant Kit provides a fast, simple, and cost-effective method for purification of total RNA from plant samples by using effective spin column and unique buffer system. The kit includes RNase-Free Filtration Column CS for homogenizing and filtering viscous plant or fungal lysates, and spin column CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes. The whole process is simple, easy and safe to operate with low toxicity. The obtained RNA has high purity and is free from protein contamination.

RNAprep Pure Plant Kitは、効果的なスピンカラムと特殊なバッファーシステムを用いることで、植物サンプルから迅速かつシンプルでコスト効率の高いtotal RNA精製法を提供します。キットには粘性植物または真菌溶解物をホモジナイズおよびろ過するためのRNaseフリーろ過カラムCSおよびシリカメンブレン技術を使用して高品質RNAを精製するためのスピンカラムCR3が含まれています。簡単であり毒性が低く安全な操作により高品質なRNAを30～40分で精製可能です。精製したRNAはタンパク質汚染が無く高純度です。サンプルが二次代謝物が豊富である場合は精製効率を高めるためにBuffer HLをTIANGENが提供いたします。
Required Reagents

β-mercaptoethanol, ethanol

β-mercaptoethanol, ethanol
Features

■ Optimized buffers for plant samples make the process more convenient.

■ Unique DNase I minimizes genomic DNA contamination.

■ Unique filtration column CS eliminates other contaminations.

■ The high-purity ready-to-use RNA is suitable for sensitive downstream applications.

■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, and no CsCl gradients centrifugation are needed, which makes the process safe and reliable.

■植物サンプル用に最適化されたバッファーによって手軽な操作です。

■特殊なDNaseIはゲノムDNAのコンタミネーションを最小限に抑えます。

■特殊なFiltration Column CSは他のコンタミネーションを排除します。

■すぐに敏感な下流の操作への使用に適した高純度のRNA

■フェノール/クロロホルム抽出、塩化リチウムによるエタノール沈殿、塩化セシウムによる密度勾配遠心法を必要としません。
Applications

■ RT-PCR.

■ Northern Blot, Dot Blot.

■ Real-Time PCR.

■ Chip analysis.

■ PolyA Screening, in vitro translation, molecular cloning.

Note:

If sample is rich in secondary metabolism, Buffer HL provided by TIANGEN could be used to achieve maximum purification efficiency.

■ RT-PCR.

■ Northern Blot, Dot Blot.

■ Real-Time PCR.

■ Chip analysis.

■ PolyA Screening, in vitro translation, molecular cloning.

Note:

If sample is rich in secondary metabolism, Buffer HL provided by TIANGEN could be used to achieve maximum purification efficiency.

Experimental Example

Material: 80 mg Atenia cordifolia leaves

Method: The total RNA of Atenia cordifolia leaves was isolated using the RNAprep Pure Plant Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.

Material: 80 mg Atenia cordifolia leaves

Method: The total RNA of Atenia cordifolia leaves was isolated using the RNAprep Pure Plant Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel.
Estimated RNA yield of various samples

Estimated RNA yield of various samples

Column-based Method

RNAprep Pure Plant Kit

Catalog number / packaging

Storage

Description

Required Reagents

Features

Applications

Experimental Example

Publication