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Column-based Method

RNA Easy Fast Plant Tissue Kit

For purification of high-quality total RNA from plant tissues

Catalog number / packaging

Cat. no

ID

No. of preps

4992880

 DP452 50
Manual Inquire Inspection report

  • Storage

    The reagents can be stored at room temperature (15-25℃). The RNase-Free DNase I should be stored at 2-8℃.

  • Description

    The RNA Easy Fast Plant Tissue Kit is a rapid RNA extraction kit for plant tissues developed based on the genomic DNA removal technology exclusively developed by TIANGEN. Toxic reagents such as β-mercaptoethanol or DTT are not needed during the operation, and the whole process of RNA extraction can be completed within 30 minutes. It is not only suitable for leaf samples of common plants such as wheat and corn, but also for polysaccharide/polyphenol-rich samples such as Malus spectabilis leaf, cotton leaf, chrysanthemum leaf, hibiscus flower, potato tuber, watermelon, cucumber, pine needle, etc.

  • Required Reagents

    RNase-Free DNase I (1500 U) (TIANGEN, Cat. no.4992232)

  • Features

    ■ Simple and fast: Total RNA extraction from plant samples can be completed within 30 minutes.

    ■ Strong compatibility: This kit is not only suitable for common stem and leaf samples, but also for polysaccharide/polyphenol-rich samples.

    ■ Safe and low toxic: Toxic reagents such as β-mercaptoethanol, DTT, chloroform, phenol are not needed.

  • Applications

    Suitable for a downstream operation, including RT-PCR, RT-qPCR, chip hybridization, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning, etc.
  • The total RNA of 65 mg leaf samples (clove leaves, hibiscus leaves, wheat leaves, rice leaves, ugli fruit leaves, Prunus cerasifera leaves, fig leaves, daylily leaves, Kerria japonica leaves), 150 mg fungus samples (Lentinus edodes) and 200 mg petal samples (day-lily petals) were extracted using RNA Easy Fast Plant Tissue Kit.
  • Agilent Bioanalyzer 2100 analysis result of total RNA from Day-lily petals

  • RNA extracted from various samples listed in the table using RNA Easy Fast Plant Tissue Kit.

    Elution volume: 50 μl; Loading volume: 2 μl.

    The electrophoresis was conducted at 6 V/cm for 15 min on a 1% agarose.

Publication

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