Column-based Method
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Features
● Simple and fast: Total RNA extraction from plant samples can be com-pleted within 30 minutes.
● Strong compatibility: This kit is not only suitable for common stem and leaf samples, but also for polysaccharide/polyphenol-rich samples.
● Safe and low toxic: Toxic reagents such as β-mercaptoethanol, DTT, chloroform, phenol are not needed.
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Description
The RNA Easy Fast Plant Tissue Kit is a rapid RNA extraction kit for plant tissues developed based on the genomic DNA removal technology exclusively developed by TIANGEN. Toxic reagents such as β-mercaptoethanol or DTT are not needed during the operation, and the whole process of RNA extraction can be completed within 30 minutes. It is not only suitable for leaf samples of common plants such as wheat and corn, but also for polysaccharide/polyphenol-rich samples such as Malus spectabilis leaf, cotton leaf, chrysanthemum leaf, hibiscus fower, potato tuber, watermelon, cucumber, pine needle, etc. -
Kit Contents
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Applications
Suitable for a downstream operations, including RT-PCR, RT-qPCR, chip hybridization, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning, etc. -
Required Reagents
RNase-Free DNase I (1500 U) (TIANGEN, Cat. no. 4992232) -
Storage Condition
The reagents can be stored at room temperature (15-30℃ ). The RNase-Free DNase I should be stored at 2-8℃. -
Important Notes
● If the subsequent experiments require high RNA purity, DNase I (provided by customer) digestion can be selectively carried out according to Step 6.
● Ethanol (96%-100%) should be added to the Buffer RW before the first use as indicated on the bottle.
● The following operations are performed at room temperature unless specified.
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Experimental Example
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