Column-based Method
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Features
● Convenient: DNA, RNA and total proteins can be purifed simultane-ously from the same sample.
● Fast: DNA/RNA/protein extraction can be completed within 1 h.
● Safe: No phenol/chloroform are required for the extraction process.
● Reliable: The purifed DNA/RNA/protein can be used in various down-stream experiments.
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Description
The DNA/RNA/Protein Isolation Kit allows rapid and simultaneous ex-traction of DNA, total RNA and total proteins from cultured animal cells or tissues, and large number of diferent samples can be processed simulta-neously. The reaction can be completed within 1 h. -
Kit Contents
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Applications
Applicable for various downstream molecular experiments such as PCR and RT-PCR, etc. -
Required Reagents
β-mercaptoethanol, ethanol -
Storage Condition
Store at room temperature (15-30℃). -
Important Notes
● Add β-mercaptoethanol (β-ME) to Buffer RL to a final concentration of 1% before use. For example, add 10 μl β-mercaptoethanol (β-ME) per 1 ml Buffer RL. Buffer RL may form precipitate during storage. If necessary, redissolve by warming it at 56°C, and then equilibrate to room temperature.
● Before use, add ethanol (96-100%) to Buffer RW, Buffer PW and Buffer GD for the working solution, as described on the tag.
● Perform all steps of the procedure at room temperature (15-30°C) if not emphasized.
● For some sensitive RNA samples, genomic DNA may need to be removed completely in the following application. Please refer the on-column DNase I digestion procedure.
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