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Magnetic Beads-based Method

Magnetic Tissue/Cell/Blood Total RNA Kit

Extract RNA from various samples such as tissue cell blood with high throughput

Catalog number / packaging

Cat. no

ID

No. of preps

4992740

 DP761 50 preps
Manual Inquire Inspection report

  • Storage

    The kit can be stored for 12 months under dry conditions at room temperature (15-25°C) for 12 months and stored at 2-8℃ for a longer time.

  • Description

    The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality total RNA from various tissues, cells and blood. The product can be perfectly matched with Kingfisher Flex 96 and TGuide S32 automated nucleic acid extractors. Magnetic beads are adsorbed, transferred and released by special magnetic rods, thus realizing the transfer of magnetic beads and nucleic acids. The whole experimental process is safe and convenient, with high purity extracted total RNA, free from contamination of genomes, proteins and other impurities. If high-throughput automated extraction is required, please consult TIANGEN for the integration plan.

  • Required Reagents

    β-mercaptoethanol, ethanol.

  • Features

    The kit can meet the requirements of manual extraction as well as batch extraction on various high-throughput platforms. The products obtained by the kit meet the requirements of downstream detection experiments and NGS analysis.

  • Applications

    RNA purified by the kit is suitable for RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.

  • Total RNA was extracted from 20 mg rat liver with TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit and the relevant products from other suppliers. 5 μl of 200 μl eluate was loaded to 1% agarose gel electrophoresis, 6 V/cm.

    Conclusion: The extraction yield, purity and stability of TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit are better than that of other suppliers.

  • Total RNA was extracted from 20 mg of rat kidney with TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit in TIANGEN TGuide S32 or Thermo Kingfisher Flex96 respectively. 5 μl of 200 μl eluate was loaded to 1% agarose gel electrophoresis, 6 V/cm. Marker: TIANGEN D15000 DNA Marker. 

    Conclusion: Magnetic Tissue/Cell/Blood Total RNA Kit has good extraction yield and purity in different automated extractors.

Publication

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