● Fast: Fewer steps, simpler operation and less time.

● Efficient: More than 85% of plasmid DNA can be extracted from bacteria.

The TIANprep Mini Plasmid Kit adopts the advanced silica membrane adsorption technology to ensure easy and fast operation. The cells are treated by alkaline lysis, and then the plasmid DNA is released and bound to silica membrane. After washing away the impurities, up to 30 μg of plasmid DNA can be efficiently extracted.

This kit provides a unique buffer formulation to remove protein impurities and other organic compounds from a maximum of 1-5 ml of bacteria medium per treatment. Higher purity of DNA can be obtained compared to traditional kits, and the DNA can be directly applied in molecular biology experiments such as enzyme digestion, transformation, sequencing and PCR, etc.

TIANprep Mini Plasmid Kit can be stored dry at room temperature (15-30°C) for up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C before use. RNase A (10 mg/ml) can be stored for 15 months at room temperature (15-30°C). After addition of RNase A, Buffer P1 is stable for 6 months at 2-8°C.

● Add the provided RNase A solution to Buffer P1 before use (use 1 vial RNase A per bottle Buffer P1), mix, and store at 2-8°C.

● Check Buffer BL, P2 and P3 before use for salt precipitation. If necessary, dissolve the buffer by warming at 37°C for 10 mins.

● Avoid direct contact of Buffer P2 and P3, immediately close the lid after use.

● All centrifugation steps are carried out at 12,000 rpm (~13,400 ×g) in a table-top microcentrifuge at room temperature (15-30°C).

● The amount of extracted plasmid is related to cells concentration and plasmid copy.

● Using Buffer BL to treat spin columns could activate silica membrane at maximum degree and higher yield.

● After treated with Buffer BL, use the Spin Column soon, otherwise long-term placement may affect the purifying effect.

For low copy plasmids and plasmids larger than 10 kb, the amount of bacteria should be increased. It is recommended to use 5-10 ml overnight culture, and the volume of Buffer P1, P2 and P3 should be increased in proportion. Buffer EB should be preheated in 65-70°C water bath, and the incubation time for adsorption and elution can be appropriately prolonged to increase the extraction efficiency. The other steps are the same as the above protocol.