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Storage
HighPure Maxi Plasmid Kit can be stored at room temperature (15-30°C) for up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers to 37°C before use. RNase A (100 mg/ml) can be stored for 15 months at room temperature (15-30°C). After adding RNase A, Buffer P1 is stable for 6 months at 2-8°C.
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Description
HighPure Maxi Plasmid Kit adopts specially designed buffer system that is used in conjunction with isopropanol precipitation to achieve fast and efficient purification of high pure plasmid DNA. Plasmid DNA prepared by HighPure Maxi Plasmid Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection to cells.
Recommended bacterial culture volume: 500-1500 μg plasmid from 100 ml bacterial culture for high-copy plasmid; and 200-400 μg plasmid from 200 ml bacterial culture for low-copy plasmid.
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Features
■ High yield: Up to 1.5 mg of plasmid DNA can be extracted by the direct precipitation of isopropanol.
■ High purity: The combination of Buffer P4 and Filtration CS1 can ensure the extraction of high-purity plasmid DNA which can meet the requirements of a variety of transfection experiments.
■ Simple and fast: The experiment can be completed in around 1 h with just a few simple centrifugation steps.
■ Wide range of applications: The purified plasmids can be applied in molecular biology experiments such as restriction endonuclease digestion, PCR, transformation, sequencing and transfection. The color indicator provided in this kit can ensure the plasmid extraction efficiency.
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Plasmid Yield
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