Single Column

EndoFree Mini Plasmid Kit II

For purification of endotoxin-free transfection grade plasmid DNA

Catalog number / packaging

Cat. no


No. of preps


DP118-02 50
  • Storage

    Room temperature (15-25℃ )
  • Description

    The EndoFree Mini Plasmid Kit II adopts a unique silica membrane adsorption technology to efficiently and specifically bind plasmid DNA. The special endotoxin-removing system can effectively remove impurities such as endotoxin and proteins. The whole extraction process takes only 1 hour, which is convenient and quick. The plasmid extraction yield is related to the plasmid copy number, host bacteria type and the culture conditions. Up to 70 μg of plasmid DNA can be extracted from 5-15 ml of the overnight cultured bacteria culture medium.
  • Required Reagents

    Ethanol, isopropanol
  • Features

    ■ Rapid and high yield: 5-70 μg plasmid DNA can be extracted in around 1 hour.

    ■ High-purity: The special endotoxin removal system combined with Spin Column CP4 can specifically adsorb nucleic acid and remove endotoxin to the minimal level.

    ■ Wide range of applications: Suitable for routine experiments such as restriction endonuclease digestion, PCR, sequencing, ligation, transformation, as well as the high-precision experiments such as gene therapy, cell microinjection, gene silencing, transcription, etc.

  • Plasmid Yield

Experimental Example

  • Suitable for the extraction of high/low copy plasmids

    Figure 1. Extraction of plasmid DNA from various volume of bacteria culture medium using EndoFree Mini Plasmid Kit II.

    Elution volume: 200 μl; Loading volume: 3 μl low-copy plasmid pBR322, 2 μl high-copy plasmid pBS.

    The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose.

    Cell transfection by the purified plasmid

  • Figure 2 The pEGFP plasmid purified by EndoFree Mini Plasmid Kit II was transfected into 293T cells. Expression of GFP were detected in 48 h post-transfection