Single Column

EndoFree Mini Plasmid Kit II

For purification of endotoxin-free transfection grade plasmid DNA

Catalog number / packaging

Cat. no	ID	No. of preps
4992422	DP118-02	50

Manual Inquire

Storage

Room temperature (15-25℃ )

Room temperature (15-25℃ )
Description

The EndoFree Mini Plasmid Kit II adopts a unique silica membrane adsorption technology to efficiently and specifically bind plasmid DNA. The special endotoxin-removing system can effectively remove impurities such as endotoxin and proteins. The whole extraction process takes only 1 hour, which is convenient and quick. The plasmid extraction yield is related to the plasmid copy number, host bacteria type and the culture conditions. Up to 70 μg of plasmid DNA can be extracted from 5-15 ml of the overnight cultured bacteria culture medium.

本キットは独自のシリカメンブレン技術を用いて、プラスミドDNAを効率的かつ特異的に結合します。同時に特殊な脱エンドトキシンシステムを採用し、エンドトキシン、タンパク質などの不純物を効果的に除去することができます。抽出の全過程は僅か1hで、簡単かつ迅速です。プラスミドの抽出収量はプラスミドのコピー数、宿主菌株の種類と培養の条件などの要因と関係があり、5-15mlの一晩に培養した細菌培養液を処理すると、70µgまでのプラスミドDNAが抽出できます。
Required Reagents

Ethanol, isopropanol

Ethanol, isopropanol
Features

■ Rapid and high yield: 5-70 μg plasmid DNA can be extracted in around 1 hour.

■ High-purity: The special endotoxin removal system combined with Spin Column CP4 can specifically adsorb nucleic acid and remove endotoxin to the minimal level.

■ Wide range of applications: Suitable for routine experiments such as restriction endonuclease digestion, PCR, sequencing, ligation, transformation, as well as the high-precision experiments such as gene therapy, cell microinjection, gene silencing, transcription, etc.

■高速かつ多収:1h前後で5-70µgプラスミドを抽出。

■高純度のプラスミド:特殊な脱エンドトキシンシステムに加えて、spin column CP4により、エンドトキシン含有量が低い。

■幅広い適用:制限酵素消化、PCR、遺伝子配列決定、連結反応、形質転換など通常の試験や遺伝子治療、微量注入、遺伝子サイレンシング、トランスフェクション実験などの高度高級の試験に適している。
Plasmid Yield

Experimental Example

Suitable for the extraction of high/low copy plasmids

Figure 1. Extraction of plasmid DNA from various volume of bacteria culture medium using EndoFree Mini Plasmid Kit II.

Elution volume: 200 μl; Loading volume: 3 μl low-copy plasmid pBR322, 2 μl high-copy plasmid pBS.

The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose.

Cell transfection by the purified plasmid

Suitable for the extraction of high/low copy plasmids

Figure 1. Extraction of plasmid DNA from various volume of bacteria culture medium using EndoFree Mini Plasmid Kit II.

Elution volume: 200 μl; Loading volume: 3 μl low-copy plasmid pBR322, 2 μl high-copy plasmid pBS.

The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose.

Cell transfection by the purified plasmid
Figure 2 The pEGFP plasmid purified by EndoFree Mini Plasmid Kit II was transfected into 293T cells. Expression of GFP were detected in 48 h post-transfection

Figure 2 The pEGFP plasmid purified by EndoFree Mini Plasmid Kit II was transfected into 293T cells. Expression of GFP were detected in 48 h post-transfection

Single Column

EndoFree Mini Plasmid Kit II

Catalog number / packaging

Storage

Description

Required Reagents

Features

Plasmid Yield

Experimental Example

Publication