● High purity: The unique endotoxin precipitation technology is adopted to specifically remove endotoxin.

● Easy to operate: The adsorption column technology is used to specifically adsorb plasmid DNA, which makes the operation easier.

● High-efficiency transfection: Suitable for the transfection of most cell lines including endotoxin-sensitive cells.

● Wide range of applications: The purified plasmid can be applied in the transfection of animal and plant cells as well as molecular biology experiments.

The EndoFree Maxi Plasmid Kit V2 adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. The special Buffer ER and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour.

Recommended bacteria culture medium amount: For high-copy plasmid, 100 ml bacteria medium is recommended, which can yield up to 500-1500 μg plasmid. For low-copy plasmid, 200 ml of bacteria medium is recommended to achieve the yield of around 50-300 μg.

The plasmid DNA extracted using this kit can be used for various regular operations, including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of various cells.

● RNase A should be added into Buffer P1 before use (add all RNase A provided in the kit). Mix well and store at 2-8°C.

● Check if there’s crystal or precipitate formed in Buffer BL, Buffer P2 or Buffer P4 before use. If crystal or precipitate are present, warm the solution in a 37°C water bath for a few minutes until the buffers turn clean.

● Be careful not to touch Buffer P2 and P4 directly, and tighten the cap immediately after use.

● When using the Filtration CS1, carefully pull the plunger out of the filter tube to avoid loosening the filter membrane due to pressure.

● The amount of the plasmid extracted is related to factors such as bacterial culture concentration and plasmid copy number. If the plasmid is low-copy or is with the size larger than 10 kb, increase the bacterial culture amount, and the amount of Buffer P1, P2 and P4 should be increased in proportion. It is recommended to preheat the Buffer TB in a 65-70°C water bath before use. The adsorption and elution time can be appropriately extended to improve the extraction efficiency.

● Prior to each experiment, please balance the spin column with Buffer BL to maximize the activation of the silicon matrix membrane and increase the yield.

● The column balanced with Buffer BL should be used immediately, otherwise the effect will be affected.

● There may be slight color changes in Buffer ER after repeated uncapping, which does not affect the final plasmid extraction efficiency and purity.