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EndoFree Maxi Plasmid Kit V2

Purification of endotoxin-free transfection grade plasmid DNA specific for sensitive cells

Catalog number / packaging

Cat. no

ID

No. of preps

4992438

 DP120-01 10
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃ )

  • Description

    The EndoFree Maxi Plasmid Kit V2 adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. The special Buffer ER and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour.

    Recommended bacteria culture medium amount: For high-copy plasmid, 100 ml bacteria medium is recommended, which can yield up to 500-1500 μg plasmid. For low-copy plasmid, 200 ml of bacteria medium is recommended to achieve the yield of around 50-300 μg.

  • Required Reagents

    Ethanol, isopropanol

  • Features

    ■ High purity: The unique endotoxin precipitation technology is adopted to specifically remove endotoxin.

    ■ Easy to operate: The adsorption column technology is used to specifically adsorb plasmid DNA, which makes the operation easier.

    ■ High-efficiency transfection: Suitable for the transfection of most cell lines including endotoxin-sensitive cells.

    ■ Wide range of applications: The purified plasmid can be applied in the transfection of animal and plant cells as well as molecular biology experiments.

  • Applications

    The plasmid DNA extracted using this kit can be used for various regular operations, including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of various cells.

  • Experimental Procedure

Experimental Example

  • The unique endotoxin removal reagent Buffer ER can efficiently remove endotoxin residues in the reaction system, and high-purity plasmid can be obtained. The plasmid endotoxin residue is ≤ 0.1 EU/μg.

  • The plasmid purified using EndoFree Maxi Plasmid Kit V2 and the same products from Supplier 1 and Supplier 2 are eluted with the same volume of elution buffer. 1 μl plasmid was loaded per lane to estimate the concentration of the plasmid. 100 ng of the plasmid whose concentration was determined by spectrophotometer was loaded into the same gel to determine whether the concentration value was falsely high.

    Conclusion: The electrophoresis result shows the actual concentration of the plasmid extracted by the EndoFree Maxi Plasmid kit V2, whether high copy or low copy, is significantly higher than that extracted by suppliers 1 and 2.

Publication

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