Single Column

TIANprep Rapid Mini Plasmid Kit

For fast purification of plasmid DNA of molecular biology grade by alkaline lysis technique

Catalog number / packaging

Cat. no

ID

No. of preps

4992192

DP105-03 200

4992191

DP105-02 50
  • Storage

    Room temperature (15-25℃ )
  • Description

    The TIANprep Rapid Mini Plasmid Kit is optimized from traditional alkaline lysis technology, by which high-quality plasmid DNA could be purified within 8 minutes. The new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt. The material which the silica membrane is made of is unique, highly efficient and highly specific. This kit is designed for purification of up to 24 μg plasmid DNA from 1-4 ml overnight cultures of E.coli. The plasmid DNA purified by this kit can be applied in various regular experiments, such as enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro transcription and the transfection of the common sub-culture cells, etc.
  • Required Reagents

    Ethanol
  • Features

    ■ Fast: Simple procedure with minimal steps to purify high pure plasmid DNA within only 8 min.

    ■ High efficiency: Could purify more than 85% plasmid DNA from E.coli.

    ■ The color indicator TIANRed was supplied to control and remind every process during the experiment to ensure the high efficiency of plasmid purification.

Experimental Example

  • Comparison between TIANprep Rapid Mini Plasmid Kit and Supplier A (general plasmid DNA purification kit) by purifying the same plasmid at the same time.

    Sample volume: 3 ml overnight culture of E.coli (OD600=1.8); Elution volume: 50 μl; Loading volume: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

    The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel. M: TIANGEN DNA Marker IV.

    Conclusion: Agarose gel electrophoresis shows that TIANprep Rapid Mini Plasmid Kit could obtain same yield of plasmid DNA as that by the general plasmid purification kit within 8 min.

  • With high-efficiency lysate treatment and optimized buffer system, TIANprep Rapid Mini Plasmid Kit can easily obtain higher quality products while saving a lot of operation time