● Fast: Simple procedure with minimal steps to purify high pure plasmid DNA within only 8 min.

● High efficiency: Could purify more than 85% plasmid DNA from E.coli.

● The color indicator TIANRed was supplied to control and remind every process during the experiment to ensure the high efficiency of plasmid purification.

The TIANprep Rapid Mini Plasmid Kit is optimized from traditional alkaline lysis technology, by which high-quality plasmid DNA could be purified within 8 minutes. The new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt. The material which the silica membrane is made of is unique, highly efficient and highly specific. This kit is designed for purification of up to 24 μg plasmid DNA from 1-4 ml overnight cultures of E.coli. The plasmid DNA purified by this kit can be applied in various routine experiments, such as enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro transcription and the transfection of the common sub-culture cells, etc.

● Add the provided RNase A and TIANRed solutions to Buffer P1 before use, mix, and store at 2-8°C.

● Check Buffer P2 and P5 before use for salt precipitation. Redissolve any precipitate by warming at 37°C.

● Avoid contacting Buffer P2 and P5 directly, and immediately close the lid after use.

● All centrifugation steps are carried out at 12,000 rpm (~13,400× g) in table-tap microcentrifuge at room temperature (15-30°C).

● The obtained plasmid amount is influenced by bacteria culture density and plasmid copy number as well.

● TIANRed user guide: TIANRed is an indicator, which is harmless and used to make sure that the whole experimental process works well. TIANRed should be mixed with Buffer P1 in the ratio of 1:200 and the color of the mixed solution should be clear red. Add the mixed solution to cell culture and the solution would turn turbid red. After that, add Buffer P2 to the turbid solution, the solution would turn clear purple which means a complete lysis. Add Buffer P5 to the purple solution and it would turn clear yellow, which indicate that the neutralization reaction has been done.