Single Column
Inquire-
Features
● Fast and high yield: 200 μg-1.5 mg plasmid DNA yield by 35 min protocol with high proportion of super-coiled structure.
● High-purity: Highly pure plasmid DNA are acquired by unique buffer system and Spin Column CP6.
● Excellent transfection efficiency: Suitable for transfection experiments of most cell lines.
● Wide range of applications: Suitable for restriction endonuclease digestion, transformation, sequencing, microinjection, gene silencing and transfection experiments.
-
Description
EndoFree Maxi Plasmid Kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt, and uses Buffer P4 and Filtration CS1 to wipe off the endotoxin and protein impurity effectively. Plasmid DNA prepared by EndoFree Plasmid Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells.
Recommended bacterial culture volume: 500-1500 μg plasmid with 100 ml bacterial culture for high-copy vectors; and 200-600 μg plasmid with 200 ml bacterial culture for low-copy vectors.
-
Kit Contents
-
Required Reagents
96-100% ethanol, isopropanol, 5M NaCl (optional) and 70% ethanol (optional)
-
Yield For Reference
-
Storage Condition
EndoFree Maxi Plasmid Kit can be stored dry at room temperature (15-30°C) for up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers to 37°C before use. RNase A (100 mg/ml) can be stored for 15 months at room temperature (15-30°C). After adding RNase A, Buffer P1 is stable for 6 months at 2-8°C.
-
Important Notes
● Add the provided RNase A solution to Buffer P1 (use 1 vial RNase A per bottle Buffer P1), mix, and store at 2-8°C.
● Check Buffer BL, P2 and P4 before use for salt precipitation. If necessary, dissolve the buffer by warming at 37°C.
● Avoid direct contact of Buffer P2 and P4, immediately close the lid after use.
● Draw out the plunger from the Filtration CS1 slowly to avoid membrane loose.
● The amount of extracted plasmid is related to cells concentration and plasmid copy. If working with low copy vectors or large plasmid (>10 kb), it may be beneficial to increase culture volume and to increase Buffer P1, P2, and P4 in proportion. Warm the Buffer TB to 65-70°C before use. Prolong adsorption and elution time properly to increase extraction efficiency.
● Use Buffer BL to treat spin columns could activate silica membrane at maximum degree and higher yield.
● After treated with Buffer BL, use the spin columns soon, since long-term placement may affect the purification effect.
-
Experimental Example
-
Sort by
-
Date
Date()
Date
Date()
Impact Factor
IF()
Impact Factor
IF()
