Single Column

EndoFree Maxi Plasmid Kit

For purification of endotoxin-free transfection grade plasmid DNA

Catalog number / packaging

Cat. no


No. of preps


DP117 10
  • Storage

    Room temperature (15-25℃ )
  • Description

    The EndoFree Maxi Plasmid Kit is based on silica membrane technology to extract endotoxin-free plasmid DNA. This kit provides a unique endotoxin removal process to generate high-quality plasmid DNA for downstream applications. It is designed to isolate plasmid DNA of the highest purity from 100-200 ml of bacterial culture and could yield up to 1.5 mg transfection grade plasmid DNA.
  • Features

    ■ Fast and high yield: 200 μg-1.5 mg plasmid DNA yield in 1 hour with high proportion of super-coiled structure.

    ■ High-purity: High-purity plasmid DNA are acquired by unique buffer system and Spin Column CP6.

    ■ Excellent transfection efficiency: Suitable for transfection experiments of most cell lines.

    ■ Wide range of applications: Suitable for restriction enzyme digestion, transformation, sequencing, microinjection, gene silencing and transfection experiments.

Experimental Example

  • High/low copy plasmid extraction

    Low-copy plasmid pBR322, extracted from 200 ml bacterial culture using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 0.6 μg/μl. High-copy plasmid pBS, extracted from 100 ml bacterial culture using EndoFree Maxi Plasmid Kit ,was eluted in 1 ml Buffer TB, with a concentration of 1.2 μg/μl.


    pBR322: 2 μl, pBS : 2 μl

  • pEGFP obtained with EndoFree Maxi Plasmid Kit were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells by TIANGEN TIANfect. Expression of GFP were detected in 24 hours after transfection.