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Single Column

Universal DNA Purification Kit

One kit, two functions. Monitoring the solution colors makes the purification simple.

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992197

 GDP214-03 200

4992196

 GDP214-02 50
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  • Features

    ● Excellent compatibility: The need for purification of DNA fragments from both gels and solutions can be fulfilled.

    ● Easy to operate: The gel can be lysed by adding the equal amount of lysis buffer and even large gel can be recovered.

    ● Stable process: The pH indicator allows the easy determination of the optimal pH for DNA binding, which improves the recovery efficiency.

  • Description

    The Universal DNA Purification Kit adopts a unique buffer system and the spin column to purify DNA from agarose gel or solution (PCR products, enzymatic reaction, etc.) rapidly and effectively. This kit is effective for any type of agarose in either TAE or TBE buffer. Buffer PC contains a pH indicator, allowing determination of the optimal pH for DNA binding. DNA fragments ranging from 100 bp to 8 kb could be effectively purified by using a specially developed spin column CB2. The purification efficiency is more than 80%. DNA fragments purified with Universal DNA Purification Kit can be used directly in downstream applications such as restriction enzyme digestion, PCR amplification, sequencing, hybridization, cloning ligation, etc.

  • Kit Contents


  • Storage Condition

    Universal DNA Purification Kit should be stored dry at room temperature (15-30℃) for  up to 15 months without showing any reduction in performance and quality. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C for 10 min before use.

  • Important Notes

    ● Buffer BL can improve the absorption capability and stability of the silica membrane, and eliminate any negative influence from environmental factors such as high temperature or humidness. Check buffers before use for salt precipitation. Redissolve any precipitate by incubating at 37°C for 10 minutes.

    ● DNA adsorption requires pH≤7.5, and the pH indicator in the Buffer PC will appear yellow in this range.

  • PH Indicator Dye

    pH Indicator Dye: Easy visual determination of optimal pH for DNA adsorption.

    DNA adsorption requires pH≤7.5, and the pH indicator in Buffer PC will appear yellow in this range. If the solution is orange or violet, add 10 μl 3M CH3COONa (pH 5.0) until the solution becomes yellow.

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