Single Column

Universal DNA Purification Kit

One kit, two functions. Monitoring the solution colors and make the purification simple.

Catalog number / packaging

Cat. no


No. of preps


DP214-03 200


DP214-02 50
  • Storage

    Room temperature (15-25℃ )
  • Description

    The Universal DNA Purification Kit adopts a unique buffer system and the spin column to purify DNA from agarose gel or solution (PCR products, enzymatic reaction, etc.) rapidly and high-effectively. This kit is effective for any type of agarose in either TAE or TBE buffer. Buffer PC contains a pH indicator, allowing determination of the optimal pH for DNA binding. DNA fragments ranging from 100 bp to 8 kb could be effectively purified by using a specially developed spin column CB2. The purification efficiency is more than 80%. DNA fragments purified with Universal DNA Purification Kit can be used directly in downstream applications such as restriction enzyme digestion, PCR amplification, sequencing, hybridization, cloning ligation, etc.
  • Features

    ■ Excellent compatibility: The needs of purification of DNA fragments from both gels and solutions can be fulfilled.

    ■ Easy to operate: The gel can be lysed by adding the equal amount of lysis buffer and even large gel can be recovered.

    ■ Stable process: The pH indicator allows the easy determination of the optimal pH for DNA binding, which improves the recovery efficiency.

Experimental Example

  • pH Indicator Dye: Easy visual determination of optimal pH for DNA adsorption. DNA adsorption requires pH ≤7.5, and the pH indicator in Buffer PC will appear yellow in this range. If the solution is orange or violet, add 10 μl 3M CH3COONa (pH 5.0) until the solution becomes yellow.