Single Column
Inquire-
Storage
Room temperature (15-25℃ ) -
Description
The TIANgel Midi Purification Kit adopts a unique adsorption column to effectively recover DNA fragments from agarose gel in either TAE or TBE buffer, while removing impurities such as proteins, ions and small primers. 100 bp-30 kb DNA fragments can be recovered with the recovery rate up to 80%. The maximal amount of DNA that can be adsorbed by each column is 10 μg. -
Required Reagents
Ethanol -
Features
■ Fast: The entire operation process is fast and convenient, and can be completed in around 10 min.
■ Diverse: Single-stranded, double-stranded DNA fragments and circular plasmid DNA can be recovered.
■ High efficiency: The unique spin column and buffers ensure maximal recovery of high-purity target DNA.
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Applications
The purified DNA can be directly used for regular experiments such as restriction enzyme digestion, PCR, sequencing, library screening, ligation, transformation, etc. -
Important Notes
■ The addition of Buffer BL can improve the adsorption capacity, the uniformity and stability of the adsorption column, and eliminate the influence of high temperature, humidity or other adverse environmental factors on the adsorption column.
■ It is recommended to use new electrophoresis buffer during electrophoresis to avoid bad performance of electrophoresis and low recovery rate.
■ If high experimental demand is required in the following steps, please use Buffer TAE.
■ When cutting the gel, the UV irradiation time should be as short as possible to avoid DNA damage.
■ If the recovery rate is low, please check the pH value after the gel is fully dissolved. If the pH value is greater than 7.5, it is recommended to add 10-30 μl of 3 M sodium acetate (pH 5.2) to the DNA containing gel solution to adjust the pH to the range of 5-7.
■ When recovering DNA fragments with the size <100 bp="" and="">10 kb, please increase the volume of the gel lysis buffer and prolong the adsorption and elution time.
■ The recovery rate is related to the initial amount of DNA and the elution volume. The less the initial amount and elution volume, the lower the recovery rate.
