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EndoFree Midi Plasmid Kit

For rapid purification of medium amount of endotoxin-free transfection grade plasmid DNA

Catalog number / packaging

Cat. no

ID

No. of preps

4992853

 DP108 10
Manual Inquire Inspection report

  • Storage

    Room temperature (15-25℃ ) in dry condition

  • Description

    The EndoFree Midi Plasmid Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA.The special Buffer EBT and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour, which is convenient and quick. 

  • Features

    ■ Suitable for extracting plasmids from 50-100 ml bacteria culture medium.

    ■ The purified plasmid contain very low endotoxin and can meet the requirements of high quality transfection.

    ■ Easy to operate with a unique and rapid endotoxin removal method.

  • Applications

    The purified plasmid DNA can be applied in molecular biology experiments and cell biology experiments such as restriction endonuclease digestion,sequencing, transformation, transfection, microinjection, etc.

  • Plasmid Yield

  • Purification Workflow

  • Plasmid pEGFP-C1 is purified by the EndoFree Midi Plasmid Kit from 50 ml overnight cultured bacteria medium. The plasmid concentration was measured by Nanodrop and the yield was calculated. The result shows that the plasmid yield by EndoFree Mini Plasmid Kit is higher than that by relevant product from Supplier A.

  • The plasmid pEGFP-C1 extracted by EndoFree Midi Plasmid Kit is transfected into 80% confluence 293T cells by TIANGEN TIANfect. Expression of GFP was detected 24-36 h after transfection. The result shows that the transfection efficiency was 80%-90%. 

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