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EndoFree Midi Plasmid Kit

For extraction ultralow endotoxin plasmid from 20-50 ml bacteria culture

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992853

 GDP108 10
Manual Inquire Inspection report

  • Features

    ● High purity and yield: For purification of ultrapure 20-250 μg plasmid DNA from 20-50 ml bacteria culture.

    ● High quality: High proportion of super-coiled structure.

  • Description

    EndoFree Midi Plasmid Kit uses unique silica membrane technology which can specifically adsorb plasmid DNA efficiently. Meanwhile, this kit also uses unique Buffer EBT and Filtration CS1 to get rid of contaminants like endotoxin and protein compounds effectively. The whole experimental procedure of plasmid DNA extraction could be finished within 1 h. Plasmid DNA prepared by EndoFree Midi Plasmid Kit is suitable for a variety of downstream applications including restriction enzyme digestion, PCR, sequencing, ligation, transformation and cell transfection.

    Cell culture volume: The recommended volume of bacterial culture for each extraction is 20-50 ml, and the yield of high-copy plasmids is generally around 80-250 μg; the yield of low-copy plasmids is generally around 20-50 μg.

  • Kit Contents

  • Yield For Reference


  • Storage Condition

    EndoFree Midi Plasmid Kit can be stored dry at room temperature (15-30°C) for up to 15 months. If any precipitate forms in the buffers, it should be dissolved by warming the buffers at 37°C for several minutes before use. RNase A can be stored for 15 months at room temperature (15-30°C). After the addition of RNase A, Buffer P1 should be stored at 2-8°C and it would be stable for 6 months.

  • Important Notes

    ● Add the provided RNase A solution to Buffer P1 (use 1 vial RNase A per bottle Buffer P1), mix, and store at 2-8°C.

    ● Add ethanol (96-100%) to Buffer PWF and Buffer MRDE before use, check bottle tag for volume.

    ● Check Buffer BL, P2, E3 and EBT before use to see if there is any precipitate formed. If necessary, dissolve the precipitate by warming at 37°C for several minutes.

    ● Avoid direct contact of Buffer P2, E3 and EBT, immediately close the lid after use.

    ● Draw out the plunger from the Filtration CS1 slowly to avoid membrane loose.

    ● The plasmid yield is related to cell concentration and copy number of plasmid. If working with low copy vectors or large plasmid (>10 kb), it may be beneficial to increase culture volume and to increase Buffer P1, P2, E3 and EBT in proportion. Warm the Buffer TB at 65-70°C before use (Prolong adsorption and elution time properly could increase extraction efficiency).

    ● Use Buffer BL to equilibrate spin columns before use could maximally activate silica membrane and increase the yield.

    ● After treatment with Buffer BL, use the spin column as soon as possible, or else the efficiency would be reduced.

    ● The collection tube (15 ml) in the kit cannot be used for the bacterial culture collection.

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