● Fast: The entire operation process is fast and convenient, and can be completed in around 10 min.

● Convenient: Gel slides can be dissolved at room temperature without the use of balance buffer.

● High efficient: The unique spin column and buffers ensure maximal recovery of high-purity target DNA.

The TIANgel Purification Kit adopts a unique adsorption column to effectively recover DNA fragments from agarose gel in either TAE or TBE buffer, while removing impurities such as proteins, ions and small primers. 100 bp-15 kb DNA fragments can be recovered with the recovery rate up to 80%. The maximal amount of DNA that can be adsorbed by each adsorption column is 10 μg.

The purified DNA can be directly used for routine experiments such as restriction enzyme digestion, PCR, sequencing, library screening, ligation, transformation, etc.

This kit can be stored dry for 15 months under room temperature (15-30℃). For long-term storage, store at 2-8℃. When stored at 2-8℃, precipitation might be formed in buffer. Place the buffers at room temperature for a period of time before use, and if necessary, incubate the buffers in a 37℃ water bath for 10 minutes to dissolve the precipitation. 

● Use fresh buffer when performing electrophoresis to avoid impacts on the electrophoresis and the recovery efficiency.

● It is better to use TAE buffer if there is high demand for following experiment.

● All centrifugation steps are performed using a benchtop centrifuge at room temperature.

● When cutting the gel, the time of ultraviolet radiation shall be kept to the shortest to prevent damage to DNA.

● Test the pH value after the gel is sufficiently dissolved if the recovery rate is low. If the pH value is greater than 7.5, you may add 10-30 µl 3 M CH₃COONa (pH 5.2) into the gel solution containing DNA until the solution pH adjusted to 5-7.

● The recovery efficiency is related to the initial amount of DNA and the elution volume. The recovery efficiency will be low if the initial DNA amount and the elution volume are small.

The concentration and purity of recovered DNA fragments can be detected by agarose gel electrophoresis and ultraviolet spectrophotometer.

DNA should have a significant absorption peak at OD₂₆₀. If the OD₂₆₀ value is 1, then it is equivalent to about 50 μg/ml double-strand DNA and 40 μg/ml single-strand DNA.

The OD₂₆₀/OD₂₈₀ ratio should be 1.7-1.9. If it is not the elution buffer but ddH₂O is used, the ratio will be low, because the pH value and the presence of ions will affect the light absorption value, but it does not mean the purity is low.