Single Column

TIANgel Purification Kit

Room temperature gel dissolution, fast and high efficient gel recovery

Catalog number / packaging

Cat. no

ID

No. of preps

4992983

DP219-02 50

4992984

DP219-03 200
  • Storage

    This kit can be stored dry for 12 months under room temperature (15-25℃ ). For longterm storage, store at 2-8℃ . When stored at 2-8℃ , precipitation might be formed in buffer. Place the buffers at room temperature for a period of time before use, and if necessary, incubate the buffers in a 37 ℃ water bath for 10 minutes to dissolve the precipitation.

  • Description

    The TIANgel Purification Kit adopts a unique adsorption column to effectively recover DNA fragments from agarose gel in either TAE or TBE buffer, while removing impurities such as proteins, ions and small primers. 100 bp-15 kb DNA fragments can be recovered with the recovery rate up to 80%. The maximal amount of DNA that can be adsorbed by each adsorption column is 10 μg.

  • Required Reagents

    Ethanol
  • Features

    ■ Fast: The entire operation process is fast and convenient, and can be completed in around 10 min.

    ■ Convenient: Gel blocks can be dissolved at room temperature without the use of balance buffer.

    ■ High efficient: The unique spin column and buffers ensure maximal recovery of high-purity target DNA.

  • Applications

    The purified DNA can be directly used for regular experiments such as restriction enzyme digestion, PCR, sequencing, library screening, ligation, transformation, etc.

  • Electrophoregram of 10 kb DNA recovery

    Different length of DNA fragments were purified by TIANgel Purification Kit and relevant products from Supplier T, Q and A. 3 μl of the 50 μl eluent was loaded per lane.

  • Electrophoregram of 5.5 kb DNA recovery

    Different length of DNA fragments were purified by TIANgel Purification Kit and relevant products from Supplier T, Q and A. 3 μl of the 50 μl eluent was loaded per lane.

  • Electrophoregram of 2 kb DNA recovery

    Different length of DNA fragments were purified by TIANgel Purification Kit and relevant products from Supplier T, Q and A. 3 μl of the 50 μl eluent was loaded per lane.

  • Electrophoregram of 300 bp DNA recovery

    Different length of DNA fragments were purified by TIANgel Purification Kit and relevant products from Supplier T, Q and A. 3 μl of the 50 μl eluent was loaded per lane.