PCR Enzyme
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Storage
Store at -20℃. -
Storage Buffer
20 mM Tris-HCl (pH8.0)
0.1 mM EDTA
1 mM DTT
100 mM KCl
50% Glycerol
Stabilizers
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Description
HotMaster Taq DNA polymerase adopts innovative synthetic affinity ligand technology, which can reversibly block enzyme activity in a temperature-dependent manner. The polymerase can reduce the generation of non-specific amplification products in the whole PCR amplification process to the greatest extent, and greatly improves the specificity of PCR reaction. The PCR product has 3'-dA overhang, which can be directly cloned with TA vector.
There are many factors that affect the specificity of PCR, including template, primer properties and quality control of reaction conditions, etc. The appearance of highly specific Taq polymerase greatly reduces the tedious condition optimization experiments, and lays a foundation for rapid and effective purification of PCR products (such as DNA product purification kit).
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Activity Definition
1 Unit (U) HotMaster Taq DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer. -
Quality Control
The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.
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Main Technical Parameters
The HotMaster Taq DNA Polymerase has 5'-3' exonuclease activity and stronger specificity, without 3'-5' exonuclease activity. The PCR product has 3'-dA overhang, which can be directly cloned with TA vector. -
Applications
It is generally used for genome amplification with high sensitivity and strong background (e.g. detection of a specific gene site or exogenous pathogen in the genome), DNA sequencing, Multi-Plex PCR, TA cloning, etc.
