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PCR Enzyme

High Affinity HotStart Taq

High affinity antibody-modified HotStart DNA polymerase

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992774

 GET108-02 500 U,5 U/ μl

4992773

 GET108-01 250 U,5 U/ μl
Manual Inquire Inspection report

  • Storage

    Store at -20℃.

  • Description

    The High Affinity HotStart Taq in this product is a mixed product of TIANGEN Taq DNA Polymerase and its monoclonal antibody, which is suitable for HotStart PCR experiments. Before the PCR reaction is heated at high temperature, the Taq monoclonal antibody will combine with Taq polymerase to inhibit its polymerase activity. The high affinity antibody in the product can ensure the complete shielding of Taq polymerase activity at room temperature, so that the whole reaction system has high specificity. In addition, Taq DNA Polymerase in this product has higher template affinity, which can improve amplification efficiency and specificity. The 3' end of PCR product produced by the kit is A, which can be directly cloned into TA vector.

  • Features

    ■ High affinity system: High Affinity HotStart Taq is a specific antibody-modified HotStart DNA polymerase with high antibody affinity; Meanwhile, Taq DNA Polymerase has high template affinity, stable amplification efficiency and high specificity. The enzyme is matched with a carefully optimized buffer system to ensure the high sensitivity of PCR reaction.

    ■ Strong stability: This product has a high success rate for experiments that cannot be completed by common DNA polymerases such as complex templates, low copy template PCR reactions and multiple PCR reactions.

    ■ Wide applications: This product is not only suitable for ordinary PCR analysis, but also suitable for quantitative PCR analysis.

  • Applications

    Regular PCR, multiplex PCR, dye-based and probe-based real-time PCR.

  • Precautions

    1.The 5×Probe qPCR Buffer is only needed for probe-based qPCR, but not for regular PCR dye-based qPCR reaction.

    2. When performing probe-based qPCR, good amplification results can be obtained using 250 nM final concentration of primer and 200 nM final concentration of probe in most systems. If it is necessary to further optimize the primer concentration, it is suggested to adjust in the range of 50-900 nM; If the probe concentration needs to be further optimized, adjust in the range of 100-500 nM.

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