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PCR Mix

2×Taq PCR MasterMix Ⅱ (without dye)

Rapid PCR premix with high efficiency and high stress resistance

Catalog number / packaging

Cat. no

ID

No. of preps

4992921

 KT211-13 20*5*1 ml

4992913

 KT211-12 5*1ml
Manual Inquire Inspection report

  • Storage

    For long-term storage, store at -20℃ . The activity will not be affected by repeated freezing and thawing. Store at 4℃ if frequent use is needed.

  • Description

    This product is a newly optimized and upgraded ready-to-use 2×PCR premix. It contains high-purity Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancer, optimizer and stabilizer. The product has the advantages of rapidness, simplicity, high sensitivity, strong specificity and good stability. It can reduce human error to the greatest extent, save operation time and reduce the probability of contamination. It is suitable for common PCR reaction, amplification of complex templates with high GC content (>60%) or secondary structure, and large-scale gene detection. The PCR product has the 3’ end dA-overhang, which can be directly used for TA cloning after purification.

  • Required Reagents

    Primers, templates

  • Features

    ■ High amplification efficiency: DNA fragments of different sizes and sources can be amplified efficiently.

    ■ High sensitivity: As low as 10 pg of target fragments can be amplified from genomic templates.

    ■ High stress resistance: For templates with high impurity content such as rough-extracted template/bacterial culture, the target fragment can be easily amplified.

    ■ Convenient for downstream applications: The amplified fragment contains the 3' end dA-overhang, which is convenient for TA cloning.

  • Applications

    PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, large-scale gene detection, semi-quantitative PCR experiments, detection of trace DNA, etc.

Experimental Example

  • High sensitivity

    Figure 3. Different concentrations of rat and human DNA fragments were amplified using TIANGEN Taq MasterMix II (A), ordinary Taq Mix of Supplier V (B) and Supplier TK (C), respectively, to detect the amplification sensitivity. The results show that TIANGEN product could amplify the target fragment from the genome template as low as 0.01 ng, and its sensitivity is better than that of the products from Supplier V and TK.

    M: TIANGEN Marker III, N: NTC

    Template input 1-8: 200 ng, 100 ng, 50 ng, 20 ng, 10 ng, 1 ng, 0.1 ng, 0.01 ng.



  • Good universality for templates from different sources and with different lengths

    Figure 2. Fragments of different sources and lengths were amplified using TIANGEN Taq MasterMix II (A) and ordinary Taq Mix of Supplier TK (B), Supplier TR (C), Supplier V (D) and Supplier G (E) respectively. The results show that the comprehensive performance of TIANGEN products is the best in terms of amplification capability, specificity and universality.

    M: TIANGEN Marker III

    1: Soybean genomic DNA template (120 bp);

    2-3: Rice genomic DNA template (694 bp, 2258 bp);

    4: Cotton genomic DNA template (200 bp);

    5: Escherichia coli genomic DNA template (2298 bp);

    6-7: Mouse genome DNA template (1 kb, 2 kb);

    8-10: Rat genomic DNA template (1 kb, 2 kb, 2080 bp);

    11-18: Human genome DNA template (300 bp, 448 bp (GC%: 74.8%), 1100 bp, 750 bp,

    1000 bp, 1090 bp (GC%: 70.4%), 2 kb, 4 kb)



  • Figure 1. Templates from different sources were amplified by TIANGEN Taq MasterMix II and the common Taq Mix from Supplier TR respectively to detect the stress resistance of the reagents. The results show that TIANGEN products can amplify the target fragments from crude genomic templates and bacterial culture, and the stress resistance is better than that of Supplier TR. A: Crude genomic template extracted by TIANGEN TIANcombi DNA Lyse&Det PCR Kit. Prp/DN: Crude extraction and detection of human blood samples. Rice: Crude extraction and detection of rice samples. B: Colony PCR. The PCR fragment is 700 bp.

    M: TIANGEN Marker III

Publication

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