20 mM Tris-HCl (pH8.0)

0.1 mM EDTA

1 mM DTT

100 mM KCl

50% Glycerol

Stabilizers

Taq DNA Polymerase is a recombinant protein with a molecular weight of 94 kDa, which has been induced and expressed from Escherichia coli cloned with Thermo aquatica DNA Polymerase gene, and then purified and separated by three steps of column purification. The enzyme has good stability and strong specificity, with no exogenous nuclease and bacterial DNA pollution, and is suitable for routine PCR amplification.

One-tube Taq MasterMix (National High-Tech Product Certification)

■ The Taq MasterMix has improved specificity and sensitivity of PCR reaction and can amplify complex templates with high GC content, secondary structure and the like. As low as 2 copies of the target template can be amplified, ensuring more accurate experimental results.

■ The unique Taq MasterMix formula makes the whole reaction system very stable, and the activity will not be affected by repeated freeze-thaw or long-term storage at 4°C.

■ The stable and efficient pre-prepared PCR mixed solution can make the operation fast and simple, greatly reducing labor intensity and sampling error. High-performance PCR enhancer and optimizer are also included in the mix, which reduces the requirements on PCR conditions.

■ This product has both dye-containing and dye-free systems. Dye-containing MasterMix products can be directly electrophoresed after PCR, without loading buffer.

The activity of 1 unit (U) Taq DNA Polymerase is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74℃ within 30 min using activated salmon sperm DNA as template/primer.

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

It is generally used for amplification, primer extension, sequencing, and A-tailing at the blunt end of DNA that’s <6 kb and has low fidelity requirements.