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Direct PCR

Blood Direct PCR Kit

Rapid amplification of the target gene directly using blood as a template without extraction

Catalog number / packaging

Cat. no

ID

No. of preps

4992529

 KG204-01 20 µl×100 rxn
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  • Storage

    This kit should be stored at 2–8℃ for 3 months. For long-term storage, please keep at -20℃ . Avoid repeated freezing and thawing.

  • Description

    This kit adopts a genetically engineered anti-inhibitor DNA polymerase to efficiently amplify single-copy genes in the human genome. The well optimized buffer system in this kit helps the polymerase strongly resist the inhibition of PCR inhibitors, thus can directly amplify DNA using blood and cultured cells as templates. This product is easy to operate and does not require complicated steps such as DNA purification or sample pretreatment.

    This kit is provided as 2×MasterMix, and the reaction can be performed by simply adding the blood template and corresponding detection primers. It can be applied on the cultured cells of mammals such as humans, mice, pigs, cattle and other species, as well as fresh or 4℃ cryopreserved whole blood, anticoagulant (EDTA, citrate, heparin), liquefied blood clots and dry blood spots stored on Whatman 903 and FTA Elute commercial cards.

  • Features

    ■ Simple and fast: PCR amplification can be directly performed using blood as template, without the need for the tedious steps of sample preparation and DNA extraction.

    ■ High purity: The skip of sample pre-treatment and DNA extraction steps can help to avoid the cross-contamination of samples.

    ■ High throughput: The PCR identification for large-scale samples can be performed by combining the kit with 96/384-well PCR plates.

    ■ Strong universality: This kit can efficiently amplify high GC fragments or fragments with complex secondary structure, and the amplification length can be up to 5 kb.

    ■ Strong stress resistance: This kit can be applied for various species and blood samples preserved in different ways.

  • Applications

    The PCR products of this kit contains "A" at the 3'-end, which can be directly used for TA vector cloning. This kit can be used for the amplification of genomic DNA fragments, high-throughput genetic analysis and genotyping (such as gene detection) analysis.

  • Using human EDTA anticoagulation as a template, 4 genes with different GC contents were amplified by Blood Direct PCR Kit . The PCR reaction system was 20 μl, and 1 μl blood was used as template.

    M: TIANGEN Marker II; 1: Fragment size 1090 bp, GC content 68.1%; 2: Fragment size 1915 bp, GC content 70.4%; 3: Fragment size 448 bp, GC content 74.8%; 4: Fragment size 1527 bp, GC content 61.5%.

    Experimental results: Blood Direct PCR Kit can effectively amplify DNA fragments with the GC content at the range of 61.5%-74.8%, suggesting it's capable to amplify high-GC fragments.

  • Using human EDTA anticoagulation as template, 5 genes with different lengths (ActB, Prp, DN1.0, Hn2.0 and Hn4.0) were amplified by Blood Direct PCR Kit. The PCR reaction system was 20 μl, and 1 μl blood was used as template.

    M: TIANGEN Marker II; 1-3: 3 different blood samples; NTC: control without primers. Experimental results: Blood Direct PCR Kit can amplify fragments with the length as long as 4 kb, suggesting it’s capable to amplify long fragments.

  • Using human EDTA anticoagulation as template, Blood Direct PCR Kit was used for PCR detection of different blood samples. The PCR reaction system was 20 μl, and 1 μl blood was used as template.

    M: TIANGEN Marker II; 1-9: the loading amount of blood is 0.1 μl, 0.2 μl, 0.3 μl, 0.4 μl, 1 μl, 2 μl, 3 μl, 4 μl and 5 μl, respectively; NTC: control with no template

    Experimental results: Blood Direct PCR Kit has strong resistance to blood and can amplify blood samples with the loading range of 0.1-5 μl.

  • Blood samples from human, rat, chicken and other species with different treatments were used as templates. Blood Direct PCR Kit was used to amplify PRNP (human, 750 bp), Actin (rat, 200 bp), and β-Actin (Chicken, 1.0 kb). The PCR reaction system was 20 μl, and 1 μl blood was used as template. M: TIANGEN Marker II.

    Experimental results: Blood Direct PCR Kit can be applied on a wide range of samples, and the direct PCR detection can be performed on blood samples from various species with different treatments.

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