The kit is a product specially developed for multiplex PCR which contains a complete set of reagents required for multiplex PCR experiments. Among them, Multi HotStart DNA Polymerase is the Taq polymerase with the best performance among all HotStart polymerases found so far. The enzyme is thermally activated by chemical modification and must be heated at 95℃ for 15 min to fully release the enzyme activity, thus ensuring extremely high specificity. The invention enables multiple pairs of PCR primers to perform good amplification in the same reaction system and multiplex PCR reactions can be performed sensitively. The enzyme has no polymerase activity at low temperature or room temperature so that the PCR system can be set up at room temperature. High purity dNTPs can further ensure the specificity of amplification. For special templates, the amplification effect can be improved by adjusting the Mg²⁺ concentration.

■ High specificity: Chemically modified hot-start enzyme with activation time up to 15 min to ensure high specificity amplification.

■ High sensitivity: Low copy amplification and high efficiency amplification of multiplex PCR.

■ Simple operation: The enzyme is inactive at low temperature and room temperature, and the reagent can be prepared at room temperature.

1 unit (U) HotStart Taq DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74℃ within 30 min using activated salmon sperm DNA as template/primer.

It has 5'-3' exonuclease activity and no 3'-5' exonuclease activity with the strongest specificity. The 3' end of PCR product is A, which can be directly used for TA cloning.

■ Multiplex PCR experiment

■ High specificity detection experiment

■ Amplification of low-copy gene

■ PCR amplification of templates with complex structures (such as genomic DNA, cDNA, etc.)