RT-PCR Kit
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Packing Size
20 μl×25 rxn -
Storage
Store at -20℃. -
Description
This product is a highly efficient, stable, fast reverse transcription premix that can remove genomic DNA contamination. 5×FastKing-RT SuperMix contains not only all reagents required for reverse transcription reaction in RT- PCR, but also thermosensitive gDNAse for efficient removal of genomic DNA. FastKing RT Enzyme is a new type of reverse transcriptase by molecular modification. It has outstanding performance in the aspects of RNA template with high GC content, complex secondary structure and stress resistance. The thermosensitive gDNase has fast effect, high efficiency and short reaction time, and will not affect cDNA quality after treating residual genomic DNA. The combination of the two enzymes enable the depletion of the residual genome and the reverse transcription reaction simultaneously, thus greatly facilitating the reverse transcription reaction. -
Features
■ Fast: One-step to complete genome removal and efficient reverse transcription within 18 minutes by only adding templates.
■ High efficiency: The reverse transcriptase is modified with a hydrophobic motif, with RT efficiency exceeding 95%.
■ Sensitive: As low as 1 ng templates can be accurately identified.
■ Simple and easy: The exclusive thermosensitive DNase has fast effect, high efficiency with shorter reaction time, and will not affect cDNA.
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Applications
The reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction, SAGE (Serial Analysis of Gene Expression), primer extension and other conventional experiments.
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