RT-PCR Kit

FastKing gDNA Dispelling RT SuperMix

gDNA removal and reverse transcription in 18 minutes

Catalog number / packaging

Cat. no	ID	No. of preps
4992251	KR118-03	1000 rxn
4992227	KR118-02	100 rxn
4992226	KR118-01	25 rxn

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Packing Size

20 μl×25 rxn

20 μl×25 rxn
Storage

Store at -20℃.

Store at -20℃.
Description

This product is a highly efficient, stable, fast reverse transcription premix that can remove genomic DNA contamination. 5×FastKing-RT SuperMix contains not only all reagents required for reverse transcription reaction in RT- PCR, but also thermosensitive gDNAse for efficient removal of genomic DNA. FastKing RT Enzyme is a new type of reverse transcriptase by molecular modification. It has outstanding performance in the aspects of RNA template with high GC content, complex secondary structure and stress resistance. The thermosensitive gDNase has fast effect, high efficiency and short reaction time, and will not affect cDNA quality after treating residual genomic DNA. The combination of the two enzymes enable the depletion of the residual genome and the reverse transcription reaction simultaneously, thus greatly facilitating the reverse transcription reaction.

FastKing gDNA Dispelling RT Super Mixは、高速で安定した効率的なcDNA合成法を提供します。合成したcDNAは2段階のリアルタイムPCRに最適です。5×FastKing-RT SuperMixには、RT-PCRに必要なすべての試薬(酵素、Rnase阻害剤、ランダムプライマー、オリゴdTプライマー、dNTP Mixture、Reaction Buffer)が含まれています。そしてcDNAに干渉することなく効率的にゲノムDNAを除去するための特殊な熱感受性DNaseが含まれています。反応は鋳型RNA及びRnase-FreeのddH₂Oを添加するだけで開始することができます。SuperMixのFastKing RT酵素は42℃、15分で高効率の逆転写を行います。特殊修飾された疎水性モチーフを用いて、FastKingRT酵素はRNAとの親和性を高め、反応の効率とスピードを促進し、高いGC含有率または複雑な2次構造を有するテンプレートの読み込みを可能にします。
Features

■ Fast: One-step to complete genome removal and efficient reverse transcription within 18 minutes by only adding templates.

■ High efficiency: The reverse transcriptase is modified with a hydrophobic motif, with RT efficiency exceeding 95%.

■ Sensitive: As low as 1 ng templates can be accurately identified.

■ Simple and easy: The exclusive thermosensitive DNase has fast effect, high efficiency with shorter reaction time, and will not affect cDNA.

■簡単なセットアップ：本製品はプレミックスであり、鋳型RNA及びddH₂Oを添加するだけで反応を開始できます。

■高い転写効率：95％を超える逆転写効率。

■短時間反応：gDNAの除去とcDNAの合成を同時に行うことができます。1ステップで42℃、15分以内に終了します。

■複雑なテンプレートへの適応：本製品は高GC含有率または複雑な2次構造を有するRNAテンプレートの逆転写が可能です。

■良好な互換性:リアルタイムPCR検出によるフォローアップにより、高感度で良好な安定性を示します。
Applications

The reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction, SAGE (Serial Analysis of Gene Expression), primer extension and other conventional experiments.

The reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction, SAGE (Serial Analysis of Gene Expression), primer extension and other conventional experiments.

Experimental Example

Experimental example 1. cDNA was synthesized using one-step reverse quantitative reagents of TIANGEN FastKing gDNA Dispelling RT SuperMix, relevant products from Supplier A and Supplier B respectively. Detect the RN5 gene of mice using TIANGEN Talent qPCR PreMix (SYBR Green), and the amplification curve, melting curve and standard curve were analyzed. The results show that TIANGEN FastKing gDNA Dispelling RT SuperMix has the highest quantitative Ct value after reverse transcription and excellent stress resistance, and has obvious advantages for templates with high impurity residues.

Experimental example 1. cDNA was synthesized using one-step reverse quantitative reagents of TIANGEN FastKing gDNA Dispelling RT SuperMix, relevant products from Supplier A and Supplier B respectively. Detect the RN5 gene of mice using TIANGEN Talent qPCR PreMix (SYBR Green), and the amplification curve, melting curve and standard curve were analyzed. The results show that TIANGEN FastKing gDNA Dispelling RT SuperMix has the highest quantitative Ct value after reverse transcription and excellent stress resistance, and has obvious advantages for templates with high impurity residues.
Experimental example 2. cDNA was synthesized using one-step reverse quantitative reagents from TIANGEN FastKing gDNA Dispelling RT SuperMix, relevant products from Supplier A and Supplier B, respectively. Detect human HsG gene using TIANGEN Talent qPCR PreMix (SYBR Green), and manually add different concentrations of genomic DNA to detect the gDNA removal ability of different reagents. Ct results show that TIANGEN FastKing gDNA Dispelling RT SuperMix has excellent ability to remove genomic DNA. Up to 500 ng of genomic DNA residue can be removed perfectly without affecting the results. ND: Not detected. NRT: Detection of the mix without reverse transcription

Experimental example 2. cDNA was synthesized using one-step reverse quantitative reagents from TIANGEN FastKing gDNA Dispelling RT SuperMix, relevant products from Supplier A and Supplier B, respectively. Detect human HsG gene using TIANGEN Talent qPCR PreMix (SYBR Green), and manually add different concentrations of genomic DNA to detect the gDNA removal ability of different reagents. Ct results show that TIANGEN FastKing gDNA Dispelling RT SuperMix has excellent ability to remove genomic DNA. Up to 500 ng of genomic DNA residue can be removed perfectly without affecting the results. ND: Not detected. NRT: Detection of the mix without reverse transcription

RT-PCR Kit

FastKing gDNA Dispelling RT SuperMix

Catalog number / packaging

Packing Size

Storage

Description

Features

Applications

Experimental Example

Publication