Dye-based qPCR
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Storage
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Description
SuperReal PreMix Plus is a special reagent for Real-Time PCR developed on the basis of SuperReal PreMix. It is simple and convenient, with unique two-component HotStart DNA polymerase, optimized buffer system with original H-bond factor and ROX packaged separately. Due to the careful optimization of buffer, the generation of primer dimer and nonspecific amplification can be more effectively reduced, ensuring higher amplification specificity and wide template adaptability. -
Features
■ Strong specificity: The buffer has a unique equilibrium system of K+ and NH4+ and an original H-Bond factor. The reaction system is upgraded and has higher amplification specificity.
■ High sensitivity: Able to quantitatively detect the expression level of transcripts with low abundance.
■ Good repeatability: The experimental results are stable and have good repeatability.
■ Simple and fast: Only template, primer and ddH2O need to be added in the premix to carry out real-time PCR reaction.
■ ROX correction: ROX dyes packaged separately are more flexible to use and can ensure more accurate results.
■ Widely applicable: It is widely applicable to experiments such as gene expression analysis and nucleic acid detection by SYBR Green method on various real-time PCR instruments such as ABI, Stratagene, Roche, Bio-Rad and Eppendorf.
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