Dye-based qPCR

SuperReal PreMix Plus (SYBR Green)

Dual-enzyme family star products with outstanding stability and specificity

Catalog number / packaging

Cat. no

ID

No. of preps

4992248

FP205-03 20 µl×5000 rxn

4992215

FP205-02 20 µl×500 rxn

4992214

FP205-01 20 µl×125 rxn
  • Storage

    Store at -20℃.
  • Description

    SuperReal PreMix Plus is a special reagent for Real-Time PCR developed on the basis of SuperReal PreMix. It is simple and convenient, with unique two-component HotStart DNA polymerase, optimized buffer system with original H-bond factor and ROX packaged separately. Due to the careful optimization of buffer, the generation of primer dimer and nonspecific amplification can be more effectively reduced, ensuring higher amplification specificity and wide template adaptability.
  • Features

    ■ Strong specificity: The buffer has a unique equilibrium system of K+ and NH4+ and an original H-Bond factor. The reaction system is upgraded and has higher amplification specificity.

    ■ High sensitivity: Able to quantitatively detect the expression level of transcripts with low abundance.

    ■ Good repeatability: The experimental results are stable and have good repeatability.

    ■ Simple and fast: Only template, primer and ddH2O need to be added in the premix to carry out real-time PCR reaction.

    ■ ROX correction: ROX dyes packaged separately are more flexible to use and can ensure more accurate results.

    ■ Widely applicable: It is widely applicable to experiments such as gene expression analysis and nucleic acid detection by SYBR Green method on various real-time PCR instruments such as ABI, Stratagene, Roche, Bio-Rad and Eppendorf.

Experimental Example

  • In some reaction systems, compared with the traditional single-enzyme amplification system, the dual-enzyme automatic regulation system has stronger fluorescence value and higher amplification efficiency.

  • Comparison of Dual HotStart enzyme automatic regulation system and traditional single enzyme system


  • Comparison of H-bond factor buffer system and common fluorescence quantitative buffer system. In the amplification experiment of hard to amplify template, the H-bond factor buffer system of SuperReal PreMix Plus has stronger reaction specificity and better repeatability than the common buffer system.

    Supplier A's buffer system


  • Comparison of H-bond factor buffer system and common fluorescence quantitative buffer system. In the amplification experiment of hard to amplify template, the H-bond factor buffer system of SuperReal PreMix Plus has stronger reaction specificity and better repeatability than the common buffer system.

    TIANGEN's H-bond factor buffer system