Dye-based qPCR

FastFire qPCR PreMix (SYBR Green)

The fastest SYBR Green fluorescent quantitative reagent

Catalog number / packaging

Cat. no


No. of preps


FP207-03 20 µl×5000 rxn


FP207-02 20 µl×500 rxn


FP207-01 20 µl×125 rxn
  • Storage

    Store in dark at -20℃.
  • Description

    This product is a special reagent for Real-Time PCR by SYBR Green I chimeric fluorescence method, which can be used for rapid and specific quantitative detection of target DNA. The optimized premix can shorten the reaction time and is suitable for standard or fast PCR instruments.

    FastFire qPCR PreMix uses antibody modified Anti Taq DNA polymerase and a unique fast PCR Buffer system to ensure sensitive qPCR reaction on all Real-Time PCR instruments. It has the characteristics of fast reaction, with 60% shorter PCR reaction time, high amplification efficiency, high amplification specificity and wide credible range, enabling faster result collection without affecting PCR effect.

  • Features

    ■ Fastest: FastFire series SYBR Green products use fast activatable antibody modified Anti Taq DNA polymerase, cooperating with unique fast Buffer system, which can save up to 60% of reaction time and become the fastest SYBR Green reagent on the market at present.

    ■ Strong amplification capability: Strong amplification fluorescence signal, more accurate and credible results.

    ■ Good stability: A unique PCR stabilizer and enhancer is added to Buffer, making the result more stable, repeatable, accurate and credible.

    ■ Widely applicable: It is not only suitable for fast real time PCR instruments, but also suitable for common real time PCR instruments.

    ■ ROX correction: ROX dye is packaged separately, which is more flexible to use and can ensure more accurate results.

Experimental Example

  • Fastest-Improve the experiment efficiency, prolong the service life of the instrument and save energy.

    The antibody modified Anti Taq DNA polymerase can be activated rapidly, ensuring FastFire products become the fastest SYBR Green reagent on the market at present with a unique fast Buffer system to improve the experimental efficiency.

    The fastest SYBR Green reagent at present, shortens the reaction time by 1000 sec.

  • Strong amplification capability- Stronger fluorescence signal

    The amplified fluorescence signal is stronger (amplification capability is strong), with a more standard amplification curve, high sensitivity, and can accurately and quantitatively detect the target gene of the low-concentration template. The detection signal of relevant product from Supplier T is weak, and the CT value is backward, which may lead to inaccurate results.

  • Better stability-Better repeatability of results

    96-well parallel repeated experiments show that the reagent has stronger stability and better repeatability.