● Highly sensitive antibody modified polymerase can shorten the reaction time by half.

● The H-Competitor competes with hydrogen bond, which ensures higher amplification efficiency of templates with high GC content, complex secondary structure and long templates.

● EP component stabilizes PCR system, effectively protects enzyme activity and resists interference of various inhibitors.

● Colorless transparent tube replaces brown tube, so that the remaining amount is visible.

This product is a special reagent for Real-Time PCR using SYBR Green I chimeric fluorescence. The 2×Talent qPCR PreMix adopts antibody-modified Anti Taq DNA polymerase, and the unique qPCR Buffer system can ensure the product to carry out highly sensitive and fast qPCR reaction on all Real-Time PCR instruments. The qPCR reaction time can be shortened by 50%. In addition, the addition of H-Competitor factor and EP component in Buffer makes the product have a wide range of sample universality. It has very good applicability to templates with complex advanced structures, templates with more PCR inhibitor residues, long fragment amplification, etc.

The product also has the characteristics of high amplification rate, high amplification specificity, wide credible range and the like, and can obtain results faster without affecting the PCR effect.

It is suitable for expression analysis, nucleic acid detection and other types of experiments on various real time PCR instruments by SYBR Green method, especially for high GC, complex secondary structure, high impurity residue and long template quantification.

Human UH11 gene (GC: 73.4%) was quantified by TIANGEN Talent qPCR PreMix (left) and relevant product from Supplier A (middle) and B (right). The results show that compared with other supliers, Talent qPCR PreMix has clear quantitative curves for 5 concentration gradients, lower Ct value, higher fluorescence value, and single melting curve peak, indicating Talent qPCR PreMix has good adaptability and amplification efficiency to high GC templates.

A total of 24 genes (GC content between 20%-73%) from different species (human, rat, mouse, rice, wheat, corn) were detected using TIANGEN Talent qPCR PreMix (SYBR Green) (4992882) and fluorescent quantification reagents from other global brands Y, T1, T2, and V1. 1 μg cDNA products reverse transcribed from RNA were diluted 5x, 50x, 500x, 5000x, and 50,000x for simultaneous amplification. Amplification specificity was determined using the first three gradient melting curves. The sensitivity of the reagents was determined using the fourth or fifth gradient melting curve. The results showed that TIANGEN Talent qPCR PreMix (SYBR Green) (4992882) had 100% amplification specificity (24/24) and 83.3% amplification sensitivity (20/24), which was significantly better than the competing products.

Using human cDNA as the template for a gene fragment (73% GC content) detection, the melting curve of TIANGEN Talent qPCR PreMix (SYBR Green) (4992882) produced the best peaks.

Using corn low abundant cDNA as the template for gene GAPDH (1 μg cDNA products reverse transcribed from RNA were diluted 5,000x, and 50,000x) detection, the melting curve showed that TIANGEN Talent qPCR PreMix (SYBR Green) (4992882) had specific and sensitive amplification based on the low abundant template.