The product adopts a unique two-component hot-start DNA polymerase to carry out PCR amplification. The PCR products are detected by the fluorescent probe added in the PCR reaction solution, and fluorescence intensity is detected the in the reaction process.

1. The double-hotstart polymerases in this product form a unique enzyme activity automatic regulation system. The automatic regulation system of enzyme activity is composed of chemically modified HotStart Taq DNA polymerase and antibody modified Anti Taq DNA polymerase, which makes SuperReal PreMix maintain the best DNA polymerase activity throughout the whole PCR reaction process. With careful optimization of Buffer system, it has the characteristics of accurate quantification, high amplification efficiency, good repeatability and wide credible range.

2. The optimized product is especially beneficial for Taq polymerase to perform its 5'-3' exonuclease activity, so as to achieve the best effect of fluorescence signal release. In addition, by the buffer optimization, SuperReal PreMix (Probe) also fully degrades the signal release of the fluorescence quenching group of the probe, so that higher signal can be obtained using same amount of template.

■ The initial denaturation condition for PCR reaction must be set at 95°C for 15 min to activate the hotstart polymerase.

■ If the reagents are not mixed evenly, the reaction performance will be decreased. When using it, please

mix it up and down and do not use the oscillator to mix it evenly. Try to avoid foam and use it after instantaneous centrifugation.

■ Good amplification results can be obtained in most systems using primer with final concentration of 300 nM and probe with the final concentration of 200 nM.

■ If the concentration of primer needs to be further optimized, it can be adjusted within the range of 50-900 nM; If the probe concentration needs to be further optimized, it can be adjusted in the range of 100-500 nM.

■ In the 20 μl reaction system, the input of genomic DNA or cDNA template generally should be less than 100 ng, and when reverse transcription product is used as template, the input should not exceed 20% of the final volume of PCR system.

■ Dual-enzyme advantage: Dual-enzyme hot-start system can ensure strong stability and more accurate data.

■ Wide linear detection range: The linear detection range can be up to 10⁷.

■ High sensitivity: Low abundance templates such as viruses and microorganisms can be detected.

■ Strong amplification capability: Stronger fluorescence signal.