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Genotyping

HRM Analysis Kit (EvaGreen）

Professional reagent for high resolution melting curve analysis

Catalog number / packaging

Cat. no

ID

No. of preps

4992873

 FP210-02 20 μl×500

4992776

 FP210-01 20 μl×125
Manual Inquire Inspection report

  • Storage

    Store in dark at -20℃.

  • Description

    This product combines the advantages of saturated dye EvaGreen and antibody modified polymerase. It is a professional kit suitable for High Resolution Melting (HRM) analysis. This product is 2×PreMix, and the preparation of PCR reaction solution is very simple and convenient. It has the characteristics of high resolution and high adaptability of templates.

    Different from SYBR Green, EvaGreen does not inhibit PCR reaction at high concentration. The binding of double-stranded PCR products with EvaGreen dye is saturated, so it is called Saturated Dye. The Dye Rearrangement phenomenon of SYBR Green will not occur and the difference of single base between amplification products can be well distinguished. The product can be used for analysis of known SNPs, screening of unknown SNPs, scanning of unknown mutant genes, methylation PCR analysis, etc.

  • Features

    ■ High resolution: Adopt EvaGreen saturated dyes, which has high resolution of melting curve in saturated state and can distinguish the variation of single base.

    ■ High specificity: Use antibody modified hotstart DNA polymerase to reduce non-specific amplification and improve specificity.

    ■ High stability: The carefully optimize Buffer system increase the stability of melting curve and improve the reliability of results.

    ■ ROX correction: ROX dye is packaged separately, which is more flexible to use and have more accurate results.

  • Applications

    ■ Known SNP typing.

    ■ Unknown SNP screening.

    ■ Unknown mutant genes scanning.

    ■ Methylation PCR analysis.

Experimental Example

  • Genomic DNA was extracted from 100 μl of human blood sample using TIANamp Blood DNA Kit. 50 ng DNA was loaded for the real time PCR detection. According to NCBI SNP library, the known SNP of FEN1 gene (RS 174538) is selected and detected using Roche LightCycle480. The results are as follows:

    The results show that HRM Analysis Kit is an ideal SNP locus analysis method with high repeatability of melting curve of the same genotype, clear resolution of different genotypes and no misjudgment.

    SNP information: ......CGGAAGAACACGTCG[A/G]CAGGAGCAGGCGCCT…… (Allele Frequency: A=0.314, G=0.686)

    Primer: For 108 bp fragment (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)

Publication

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