Genotyping

TIANexact Genotyping qPCR PreMix(Probe)

Probe reagent for accurate typing of SNP site

Catalog number / packaging

Cat. no

ID

No. of preps

4992875

FP211-02 20 µl×500 rxn

4992874

FP211-01 20 µl×125 rxn
  • Storage

    Store in dark at -20℃.
  • Description

    This product is a ready-to-use 2×hot-start PCR premix reagent specially applied to probe SNP detection. The premixed reagent contains specific antibody modified Taq DNA polymerase and can effectively detect extremely low abundance DNA templates. Specially prepared unique PCR buffer can effectively eliminate numerous inhibitions to PCR and influence on fluorescence signal quenching, and has good specificity and amplification efficiency for SNP identification, especially for crude samples, which shows high stress resistance. It is suitable for crude extracts of plants, animals and some environmental samples. The buffer is premixed with blue indicator dye, which has no effect on PCR reaction and probe fluorescence. It makes the operation more convenient and avoids the sample adding errors generated during the operation of a large number of small sample systems (10 μl systems).
  • Features

    ■ High specificity: Specific antibody modified hot-start Taq DNA polymerase reduces non-specific amplification and improves specificity.

    ■ Effective genotyping: Unique Buffer system can effectively shield the influence of PCR inhibitors on SNP typing.

    ■ High accuracy: Strong SNP typing fluorescence signals make the results easier to determine.

    ■ Wide application: Applicable to plant, animal and other samples, also applicable to the detection of crude extract of some environmental samples.

    ■ Blue indicator: The premixed reagent contains blue indicator dye to avoid sample loading errors.

    ■ ROX correction: ROX dye is packaged separately, which is more flexible to use and have more accurate results.

  • Applications

    Known SNP typing.

Experimental Example

  • A.    Compared with Competitor I, TIANexact shows stronger fluorescence signal and tighter clustering effect under the condition of low concentration template.
  • B. When treated with humic acid (50 ng/μl), the PCR amplification of Competitor I is completely inhibited, while TIANexact still show accurate results.