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Dye-based qPCR

SuperReal PreMix Color (SYBR Green）

Considerate and specifi c dye fl uorescence quantitative reagent

Catalog number / packaging

Cat. no

ID

No. of preps

4992902

 FP215-03 20 µl×5000 rxn

4992876

 FP215-02 20 µl×500 rxn

4992777

 FP215-01 20 µl×125 rxn
Manual Inquire Inspection report

  • Storage

    Store in dark at -20℃ .

  • Description

    The product is a special reagent for real-time PCR using SYBR Green I chimeric fluorescence. The product is a 2× concentration PreMix mixed with the optimum concentration of SYBR Green I required for real-time PCR reaction. The indicator is additionally added to the product, which is beneficial to the loading of a large number of samples and reduces the probability of misoperation.

    SuperReal Color PreMix adopts a unique two component hot start DNA polymerase (chemically modified HotStart Taq DNA polymerase and antibody modified Anti Taq DNA polymerase), and is characterized by high amplification efficiency, high amplification specificity and wide credible range in combination with carefully optimized buffer system. The upgraded reagent has more prominent advantages in amplification specificity after component adjustment.

  • Features

    ■ Color indicators are added to the reagents to avoid sample addition errors or omission due to the large number of samples.

    ■ High fluorescence value, high sensitivity and high specificity.

    ■ After strict detection, the indicator will not affect the fluorescence value and quantitative results of the dye.

    ■ Suitable for a variety of common real time PCR instruments (Roche, Bio-rad, ABI, etc.).

  • Applications

    It is suitable for various types of experiments such as expression analysis and nucleic acid detection by SYBR Green method on various real time PCR instruments. It is especially suitable for high throughput experiments with a large number of samples and complicated sample loading operation.

  • Figure. 1. Graphs of amplifi cation curves and melting curves obtained by respectively quantifying human HS1 gene using TIANGEN SuperReal PreMix Plus (SYBR Green)(A) and SuperReal PreMix Color (SYBR Green)(B). The template is diluted for 5 concentration gradients. The Ct values (C) obtained by comparison show that the amplification of the two kits have considerable sensitivity, specificity and stability. The experiment was run on Roche LightCycler480.

  • Figure. 1. Graphs of amplifi cation curves and melting curves obtained by respectively quantifying human HS1 gene using TIANGEN SuperReal PreMix Plus (SYBR Green)(A) and SuperReal PreMix Color (SYBR Green)(B). The template is diluted for 5 concentration gradients. The Ct values (C) obtained by comparison show that the amplification of the two kits have considerable sensitivity, specificity and stability. The experiment was run on Roche LightCycler480.

  • Figure. 1. Graphs of amplifi cation curves and melting curves obtained by respectively quantifying human HS1 gene using TIANGEN SuperReal PreMix Plus (SYBR Green)(A) and SuperReal PreMix Color (SYBR Green)(B). The template is diluted for 5 concentration gradients. The Ct values (C) obtained by comparison show that the amplification of the two kits have considerable sensitivity, specificity and stability. The experiment was run on Roche LightCycler480.

  • Figure 2. Graphs of amplification curve and melting curve obtained by respectively quantifying human HS5 gene using TIANGEN SuperReal PreMix Color (SYBR Green) and relevant product from Supplier T (A: TIANGEN, B: Supplier T. Dilute the template for 5 concentration gradients, and compare the Ct values (C). The result shows that the SuperReal PreMix Color (SYBR Green) has higher sensitivity, specificity and stability. The experiment was run on Bio-Rad CFX96.

  • Figure 2. Graphs of amplification curve and melting curve obtained by respectively quantifying human HS5 gene using TIANGEN SuperReal PreMix Color (SYBR Green) and relevant product from Supplier T (A: TIANGEN, B: Supplier T. Dilute the template for 5 concentration gradients, and compare the Ct values (C). The result shows that the SuperReal PreMix Color (SYBR Green) has higher sensitivity, specificity and stability. The experiment was run on Bio-Rad CFX96.

  • Figure 2. Graphs of amplification curve and melting curve obtained by respectively quantifying human HS5 gene using TIANGEN SuperReal PreMix Color (SYBR Green) and relevant product from Supplier T (A: TIANGEN, B: Supplier T. Dilute the template for 5 concentration gradients, and compare the Ct values (C). The result shows that the SuperReal PreMix Color (SYBR Green) has higher sensitivity, specificity and stability. The experiment was run on Bio-Rad CFX96.

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