Probe-based qPCR

SuperReal PreMix Color (Probe)

Stable and effi cient probe fl uorescence quantitative reagent

Catalog number / packaging

Cat. no

ID

No. of preps

4992883

FP216-01 20 µl×125 rxn

4992884

FP216-02 20 µl×500 rxn
  • Storage

    Store in dark at -20℃ .
  • Description

    This product is a special reagent supplied as 2×PreMix for Real-Time PCR by probe method. The preparation of reaction solution is very simple and convenient. The indicator is additionally added to the product, which is beneficial for adding of a large number of samples and reduces the probability of mis-operation. SuperReal Color PreMix adopts a unique two-component hot-start DNA polymerase (chemically modified HotStart Taq DNA polymerase and antibody modified Anti Taq DNA polymerase), in combination with carefully optimized buffer system, the product has accurate quantification, high amplification efficiency, good repeatability and wide credible range.

  • Features

    ■ Color indicators are added to the reagents to avoid sample addition errors or omission due to the large number of samples.

    ■ The SuperReal PreMix Color has high fluorescence value, high sensitivity and high specificity.

    ■ Suitable for many common fluorescent probes (FAM, HEX, NED, etc.). The indicator will not affect the fluorescence of the probe.

    ■ Suitable for a variety of common real time PCR instruments (Roche, Bio-rad, ABI, etc.).

  • Applications

    It is suitable for various types of experiments such as expression analysis and nucleic acid detection by probe method on various real time PCR instruments. It is especially suitable for high throughput experiments with a large number of samples and complicated sample loading.

  • Figure 1. Graphs of amplification curves and standard curves obtained by respectively quantifying human HSUBC gene using TIANGEN SuperReal PreMix Plus (Probe)(A) and SuperReal PreMix Color (Probe)(B). The template is diluted for 5 concentration gradients. The Ct values (C) show that the amplification of the two kits has considerable sensitivity and stability. The instrument used is ABI 7500 and the probe fluorescent group is FAM.
  • Figure 1. Graphs of amplification curves and standard curves obtained by respectively quantifying human HSUBC gene using TIANGEN SuperReal PreMix Plus (Probe)(A) and SuperReal PreMix Color (Probe)(B). The template is diluted for 5 concentration gradients. The Ct values (C) show that the amplification of the two kits has considerable sensitivity and stability. The instrument used is ABI 7500 and the probe fluorescent group is FAM.
  • Figure 1. Graphs of amplification curves and standard curves obtained by respectively quantifying human HSUBC gene using TIANGEN SuperReal PreMix Plus (Probe)(A) and SuperReal PreMix Color (Probe)(B). The template is diluted for 5 concentration gradients. The Ct values (C) show that the amplification of the two kits has considerable sensitivity and stability. The instrument used is ABI 7500 and the probe fluorescent group is FAM.