The kit provides all reagents for synthesizing the first strand of cDNA, wherein TIANScript II RTase is an improved Moloney murine leukemia virus reverse transcriptase (M-MLV), and the improved new RTase has higher template affinity and lower RNase H activity, thus enabling it to read through complex secondary structure templates and reverse transcript long fragment cDNA in the synthesis process of the first strand of cDNA. The 5×TIANScript II RTase Buffer provided in the product has been carefully optimized, which not only ensures the high-efficiency enzyme activity of the new RTase, but also widens the input range of RNA template amount, making the reverse transcribed cDNA have better quality for subsequent experimental analysis.

■ High enzyme activity and efficiency: High reverse transcriptase activity and good compatibility in subsequent experiments.

■ Wide substrate range: Suitable for all RNA, especially RNA templates with complex secondary structures.

■ Long RT length: The synthesis of the cDNA first strand can reach 12 kb.

■ Simple operation: Simply add the required components in one step without adding any reagent during the operation.

■ Synthesis of cDNA first strand.

■ Construction of cDNA library.

■ One-step RT-PCR.

■ RACE analysis.