Menu
English

English

Janpanese(Japan)

Korean(Korea)

RT-PCR Kit

TIANScriptⅡ RT Kit

Suitable for templates with complex secondary structures and efficient synthesis of long-chain cDNA

Catalog number / packaging

Cat. no

ID

No. of preps

4992911

 KR107-02 20 µl×100 rxn

4992910

 KR107-01 20 µl×25 rxn
Manual Inquire Inspection report

  • Storage

    Store at -20°C.

  • Description

    The kit provides all reagents for synthesizing the first strand of cDNA, wherein TIANScript II RTase is an improved Moloney murine leukemia virus reverse transcriptase (M-MLV), and the improved new RTase has higher template affinity and lower RNase H activity, thus enabling it to read through complex secondary structure templates and reverse transcript long fragment cDNA in the synthesis process of the first strand of cDNA. The 5×TIANScript II RTase Buffer provided in the product has been carefully optimized, which not only ensures the high-efficiency enzyme activity of the new RTase, but also widens the input range of RNA template amount, making the reverse transcribed cDNA have better quality for subsequent experimental analysis.

  • Features

    ■ High enzyme activity and efficiency: High reverse transcriptase activity and good compatibility in subsequent experiments.

    ■ Wide substrate range: Suitable for all RNA, especially RNA templates with complex secondary structures.

    ■ Long RT length: The synthesis of the cDNA first strand can reach 12 kb.

    ■ Simple operation: Simply add the required components in one step without adding any reagent during the operation.

  • Applications

    ■ Synthesis of cDNA first strand.

    ■ Construction of cDNA library.

    ■ One-step RT-PCR.

    ■ RACE analysis.

  • Reverse transcription ability of TIANScript II RT Kit for different length fragments


    Method: Reverse transcription: Refer to the instruction manual of TIANScript II RT Kit.

    Results: The gel picture shows the amplification results of 10 target genes with different lengths after reverse transcription of 1 μg of total RNA. 2 μl of reverse transcription products were loaded per lane.

    Amplification system (PCR): 20 μl; Sample load: 5 μl;

    Marker: D15000+1 kb DNA Ladder; Gel concentration: 1%;

    Electrophoresis conditions: 6 V/cm, 20 min

    Diagram of each lane: M: DNA Marker; 1: Product length: 120 bp; 2: Product length: 1 kb; 3: Product length: 2.5 kb; 4: Product length: 3.2 kb; 5: Product

    length: 4.6 kb; 6: Product length: 6.8 kb; 7: Product length: 7.6 kb; 8: Product length: 8.9 kb; 9: Product length: 10 kb; 10: Product length: 12 kb;


  • Comparison of efficiency and specificity of TIANScript II RT Kit and products from other suppliers in reverse transcription of long templates


    Materials: Total RNA of human adherent cells.

    RT-PCR starting amount: 2 μl reverse transcription product (50 ng/μl)

    Method: Reverse transcription: Refer to to the instruction manual of TIANScript II RT Kit.

    Results: The gel picture shows the amplification results of 6 target genes with different lengths after reverse transcription of 1 μg total RNA of human adherent cells using M-MLV from Supplier A and TIANGEN TIANScript II RT Kit.

    Amplification system (PCR): 20 μl; Sample load: 5 μl;

    Marker: DNA MarkerIII; Gel concentration: 1%;

    Electrophoresis conditions: 6 V/cm, 20 min.

    Diagram of each lane: M: DNA Marker; 1: Amplification results of the cDNA reverse transcribed using TIANScriptII RT Kit; 2. Amplification results of the cDNA reverse transcribed using relevant product from Supplier A. The length of gene 1 product is 1.3 kb; The length of gene 2 product is 3.0kb; The length of gene 3 product is 5.0 kb; The length of gene 4 product is 7.5 kb.



Publication

Inquire