Quantscript RT Kit (cDNA first strand synthesis kit) is specially prepared for the first step of two-step RT-PCR experiment. The product comprises a brand-new high-efficiency reverse transcriptase Quant Reverse Transcriptase, a reaction buffer and necessary reaction components for RT reaction. Total RNA or mRNA can be used as a template for efficient reverse transcription into complementary cDNA first strand with this kit, and further downstream experiments can be carried out.

■ The reverse transcription efficiency can reach 90%.

■ The whole experiment can be completed in one step at 37℃ .

■ RNA template with high GC content and complex secondary structure can be read through.

■ It has good compatibility with subsequent PCR or qPCR experiments and is compatible with various PCR thermostable polymerases.

■ Suitable for reverse transcription of total RNA template with amount of 50 ng-2 μg.

■ Real-time RT-PCR.

■ Semi-quantitative PCR reaction.

■ 3'- and 5'- RACE, etc.