QuantScript RT Kit

Rapid and efficient reverse transcription reaction on 50 ng-2 μg of total RNA

Catalog number / packaging

Cat. no


No. of preps


KR103-03 20 µl×25 rxn


KR103-04 20 µl×100 rxn
  • Storage

    Store at -20℃ .
  • Description

    Quantscript RT Kit (cDNA first strand synthesis kit) is specially prepared for the first step of two-step RT-PCR experiment. The product comprises a brand-new high-efficiency reverse transcriptase Quant Reverse Transcriptase, a reaction buffer and necessary reaction components for RT reaction. Total RNA or mRNA can be used as a template for efficient reverse transcription into complementary cDNA first strand with this kit, and further downstream experiments can be carried out.

  • Features

    ■ The reverse transcription efficiency can reach 90%.

    ■ The whole experiment can be completed in one step at 37℃ .

    ■ RNA template with high GC content and complex secondary structure can be read through.

    ■ It has good compatibility with subsequent PCR or qPCR experiments and is compatible with various PCR thermostable polymerases.

  • Applications

    ■ Suitable for reverse transcription of total RNA template with amount of 50 ng-2 μg.

    ■ Real-time RT-PCR.

    ■ Semi-quantitative PCR reaction.

    ■ 3'- and 5'- RACE, etc.