DNA Library Kits/Modules

TIANSeq DirectFast Library Kit(illumina)

New generation of DNA library construction technology without fragmentation pretreatment

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992260

GNG101-02 96 rxn

4992259

GNG101-01 24 rxn
  • Storage

    Store at-15 to-25°C
  • Sample Input

    1 ng ~ 1 μg DNA
  • Description

    TIANSeq DirectFast Library Kit (Illumina) is a DNA library construction kit specifically optimized for the Illumina high-throughput sequencing platform. The kit can perform one-step DNA fragmentation, end repair and dA-tailing in one tube. The obtained product can be directly used for adapter ligation without purification. The PCR amplification reagent provided by the kit is also specially optimized to ensure the DNA sequence obtained by amplification has high yield, good fidelity and no base bias. The kit adopts a one-step reaction process, which does not need multiple purification steps, and the whole library construction process only takes 2.5 h; The conversion efficiency of the library is higher, and the high-efficiency library construction can be ensured for trace DNA samples.

  • Required Reagents

    TIANSeq Single-Indexed Adapter(Illumina) (Cat# 4992641/4992642/4992378).

    TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)

  • Features

    ■ No base bias of the DNA fragmentation process and PCR amplification process. Good sequencing uniformity.

    ■ High library conversion efficiency, and the DNA sample input can be as low as 1 ng.

    ■ The one-tube enzymatic reaction is simple and convenient to operate, which does not need multi-step purification steps. The whole library construction process only needs 2.5 hours.

  • Applications

    DNA library construction for Illumina high-throughput sequencing platform

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