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DNA Library Kits/Modules

TIANSeq DirectFast Library Kit(illumina)

New generation of DNA library construction technology without fragmentation pretreatment

Catalog number / packaging

Cat. no

ID

No. of preps

4992260

 NG101-02 96 rxn

4992259

 NG101-01 24 rxn
Manual Inquire Inspection report

  • Storage

    Store at-15 to-25°C

  • Sample Input

    1 ng ～ 1 μg DNA

  • Description

    TIANSeq DirectFast Library Kit (Illumina) is a DNA library construction kit specifically optimized for the Illumina high-throughput sequencing platform. The kit can perform one-step DNA fragmentation, end repair and dA-tailing in one tube. The obtained product can be directly used for adapter ligation without purification. The PCR amplification reagent provided by the kit is also specially optimized to ensure the DNA sequence obtained by amplification has high yield, good fidelity and no base bias. The kit adopts a one-step reaction process, which does not need multiple purification steps, and the whole library construction process only takes 2.5 h; The conversion efficiency of the library is higher, and the high-efficiency library construction can be ensured for trace DNA samples.

  • Required Reagents

    TIANSeq Single-Indexed Adapter(Illumina) (Cat# 4992641/4992642/4992378).

    TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)

  • Features

    ■ No base bias of the DNA fragmentation process and PCR amplification process. Good sequencing uniformity.

    ■ High library conversion efficiency, and the DNA sample input can be as low as 1 ng.

    ■ The one-tube enzymatic reaction is simple and convenient to operate, which does not need multi-step purification steps. The whole library construction process only needs 2.5 hours.

  • Applications

    DNA library construction for Illumina high-throughput sequencing platform

Experimental Example

  • Flexible sample input and fragmented size

    Figure 1. DNA fragmentation profiles of different reaction time. 10 ng and 1000 ng DNA were fragmented using TIANSeq DirectFast DNA Library Kit. The reaction products treated with different reaction time were purified by 1.8× Ampure XP magnetic beads and analyzed by Angilent 2100.


  • Comparison of Effect between Fragmentation Enzyme and Mechanical Fragmentation

    Figure 2. Comparison of genome coverage of different library preparation methods. Three bacterial genomic DNA with different GC contents are mixed equimolar, and sequencing genome coverage result of 100 ng of mixed DNA libraries using these methods were compared. The results show that the TIANSeq DirectFast Library Kit has the same effect on DNA fragmentation as mechanical shearing, and there is no base bias for fragmentation.

  • Comparison of Library Construction Effects with Samples as Low as 1 ng

    Figure 3. Comparison of genome coverage of different library preparation methods. Three bacterial genomic DNA with different GC contents are mixed equimolar, and sequencing genome coverage result of 1 ng of mixed DNA libraries using these methods were compared. The results show that the TIANSeq DirectFast Library Kit has consistent fragmentation effect with the mechanical shearing even for DNA input as low as 1 ng, and there is no base bias.


  • Comparison of Sequencing Results by PCR-free Procedure

    Figure 4. Different input of genomic DNA were used to construct the library by PCR or PCR-free library construction, and the genome coverage results were compared. The results show that with the one-tube operation and efficient library construction steps, the DNA library constructed with TIANSeq DirectFast Library Kit maintains a high consistency with the mechanical shearing in fragment sequence coverage distribution for both PCR enrichment PCR-free workflow.


  • Statistics of Library Construction Efficiency and Yield

    Figure 5. Results of quantitative analysis of library DNA obtained by qPCR after library construction by PCR-free method for samples with different starting amounts (1, 10, 25, 50, 100, 500,1000 ng). Linear regression analysis shows that the library yield has a good linear relationship in a wide sample input range. For DNA input as low as 1 ng, the efficiency of library construction does not decrease.

  • Comparison of Sequencing Data of Different Products


Publication

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