StorageThis kit should be stored at-25~-15°C.
DescriptionTIANSeq End Repair/dA-Tailing Module is an optimized premixed enzyme module specially designed for the construction of DNA library for Illumina high-throughput sequencing platform. It can repair the ends of small fragments of DNA or fragmented double-stranded DNA treated by ultrasonic treatment, chemical treatment and enzyme treatment to form flat ends of DNA double strands. The 5'-P and 3'-dA are added to both ends of the fragmented DNA respectively, and the obtained product is directly used for adapter ligation with TIANseq Fast Ligation Module (4992354/4992355) without purification. The kit adopts a one-step reaction process, which does not need multiple purification steps, and can carry out efficient and rapid end repair and dA-tailing on extremely low DNA samples. It is simpler and more convenient to operate and has higher library conversion efficiency.
TIANSeq Fast Ligation Module (Cat# 4992354/4992355)
TIANSeq NGS Library Amplification Module (Cat #4992373/4992374)
TIANSeq Single-Index Adapter (Illumina) (Cat# 4992641/4992642/4992378)
TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)
■ The one-tube enzymatic reaction completes the end repair and dA-tailing of double-stranded DNA fragments in one step.
■ This kit has high library conversion efficiency, and the DNA sample input can be as low as 0.25 ng.
ApplicationsThis kit is used for end repair of double-stranded DNA fragments and dA-tailing at the 3' end, and is suitable for Illumina high-throughput sequencing platform. Applicable sample input: 0.25 ng~1 μg DNA