● Simple and rapid fragmentation of double-stranded DNA can be achieved by enzymatic reaction without using special equipment.

● DNA fragmentation size can be flexibly adjusted by simply adjusting the fragmentation time.

● High reaction efficiency: The fragmentation of different input DNA samples in the range of 1 ng to 1 μg can all be completed in a single reaction system.

The TIANSeq DNA Fragmentation Module is supplied as a supporting kit for the NGS library construction of the high-throughput sequencing platform, which fragmentates large DNA fragments to desired sizes before library construction. The module performs enzymatic-based reaction to randomly cleaves double-stranded DNA into 200-500 bp fragments in tunable reaction time. The DNA fragments generated by the module are blunt-end double-stranded DNA fragments with 5'-P at the 5'-end group and 3'-OH at the 3'-end group, where dA or adapters can be directly added subsequently. The double-stranded DNA is proven to be randomly cleaved by the TIANSeq DNA Fragmentation Module. The product shows no base preference and the sequencing coverage of the fragmented DNA from different sources is consistent with that of mechanical shearing fragments. 

Ideal choice for generating double-stranded DNA fragments of tunable sized for use in NGS library construction workflow.

● TIANSeq End Repair/dA-Tailing Module (Cat# 4992352/4992353)

● TIANseq Fast Ligation Module (Cat# 4992354/4992355)

● TIANSeq NGS Library Amplification Module (Cat# 4992373/4992374)

● TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359/4992979)

The kit should be stored at -30~-15°C. Avoid repeated freezing and thawing. The shelf life is one year.

● Attention should be paid in the operating process to avoid cross-contamination between nucleic acid samples and products.

● Please use RNase- or DNase-free pipette tips or EP tubes for the experiment.

● Clean the working area before the test to ensure no contamination of RNase and DNA.

● Before proceeding related operation, make sure the thermal cycler is calibrated and in a stable state.

● Please read the protocol carefully before the experiment. If test suspension is needed or the downstream test is not needed to be carried out immediately, the test products can be frozen and stored at -20°C and the subsequent test can be planned accordingly.