DNA Library Kits/Modules
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Storage
This kit should be stored at-25~-15°C. -
Description
TIANSeq DNA Fragmentation Module is a kit designed for the construction of high-throughput sequencing platform library, which is used for fragmentation of large fragment DNA before library construction. The module adopts the principle of time-dependent enzymatic reaction to randomly cut double-stranded DNA into 200~500 bp fragments according to different reaction time. The obtained fragments are blunt-ended double-stranded DNA fragments, the 5' end group contains 5'-P, and the 3' end group contains 3'-OH, so that subsequent dA-tailing or adapter ligation can be directly performed. Random fragmentation of double-stranded DNA by TIANSeq DNA Fragmentation Module shows that the product has no base preference, and the sequencing coverage rate of different types of fragmented DNA is consistent with that of mechanical shearing. -
Required Reagents
TIANSeq Fast Ligation Module (Cat# 4992354/4992355)
TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)
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Features
■ No special instruments and equipment are needed. The double-stranded DNA can be easily and rapidly fragmented through enzymatic reaction.
■ Flexible adjustment of DNA fragmentation length by simply adjusting fragmentation time.
■ Efficient reaction: Fragmentation of 1 ng~1 μg DNA samples can be completed in a single reaction system.
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Applications
Prepare fragmented double-stranded DNA for NGS library construction
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