DNA Library Kits/Modules

TIANSeq DNA Fragmentation Module

Efficient and rapid enzyme-based fragmentation of double-stranded DNA

Catalog number / packaging

Mat. No	Ref. No	No. of preps
4992356	GNG305-01	24 rxn
4992357	GNG305-02	96 rxn

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Storage

This kit should be stored at-25~-15°C.

This kit should be stored at-25~-15°C.
Description

TIANSeq DNA Fragmentation Module is a kit designed for the construction of high-throughput sequencing platform library, which is used for fragmentation of large fragment DNA before library construction. The module adopts the principle of time-dependent enzymatic reaction to randomly cut double-stranded DNA into 200~500 bp fragments according to different reaction time. The obtained fragments are blunt-ended double-stranded DNA fragments, the 5' end group contains 5'-P, and the 3' end group contains 3'-OH, so that subsequent dA-tailing or adapter ligation can be directly performed. Random fragmentation of double-stranded DNA by TIANSeq DNA Fragmentation Module shows that the product has no base preference, and the sequencing coverage rate of different types of fragmented DNA is consistent with that of mechanical shearing.

TIANSeq DNA Fragmentation Moduleは、ハイスループットの遺伝子配列決定プラットフォームでライブラリー構築に関わるキットであり、ライブラリー構築前に大きい断片DNAを断片させます。モジュールは時間依存型の酵素反応原理に基づいて、異なる作用時間によって二本鎖DNAをランダムに200～500 bpの断片に切断し、得られた断片は平滑末端にする二本鎖DNA断片であり、5’末端基は5’−Pがあり、3’末端基は3’−OHを含有するため、後続のdAやadapter添加を直接行うことができます。TIANSeq DNA断片化モジュールによる二本鎖DNAに対するランダムな切断の結果から見ると、本製品は塩基選好性がなく、異なる種類のDNA断片を切断する遺伝子配列決定の被覆率が機械的な切断と一致していることを示唆しています。
Required Reagents

TIANSeq Fast Ligation Module (Cat# 4992354/4992355)

TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)

TIANSeq Fast Ligation Module (Cat# 4992354/4992355)

TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)
Features

■ No special instruments and equipment are needed. The double-stranded DNA can be easily and rapidly fragmented through enzymatic reaction.

■ Flexible adjustment of DNA fragmentation length by simply adjusting fragmentation time.

■ Efficient reaction: Fragmentation of 1 ng~1 μg DNA samples can be completed in a single reaction system.

■特殊な器械設備を必要とせず、酵素反応により簡便かつ迅速に二本鎖DNAを断片化する。

■柔軟に使用でき、断片化時間を調整することで、DNA断片化の長さが調整できる。

■反応が効率的で、単一の反応系で1 ng ～ 1 µg のDNAサンプルの断片化ができる。
Applications

Prepare fragmented double-stranded DNA for NGS library construction

Prepare fragmented double-stranded DNA for NGS library construction

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DNA Library Kits/Modules

TIANSeq DNA Fragmentation Module

Catalog number / packaging

Storage

Description

Required Reagents

Features

Applications

Publication