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Storage
This kit should be stored at-15~-25°C. -
Description
TIANSeq rRNA Depletion Kit (H/M/R) uses a specially designed DNA probe to hybridize with ribosomal RNA(rRNA), and then uses Ribo RNase H to degrade rRNA in the DNA-rRNA hybrid chain, thus removing cytoplasmic ribosomal RNA(28S, 18S, 5.8S, 5S) and mitochondrial ribosomal RNA(16S, 12S) in human, mouse and rat total RNA, and retaining messenger RNA(mRNA) and other non-coding RNA.
The kit has a good rRNA removal effect on complete and partially degraded total RNA (such as FFPE RNA).The obtained rRNA-removed RNA sample can be used for mRNA and non-coding RNA high-throughput sequencing. The effective data ratio in sequencing results can be obviously improved, and the purified product can also be used for random primer cDNA synthesis or other downstream applications.
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Required Reagents
TIANSeq RNA Clean Beads (Cat#4992360/4992362/4992867)
Ethanol
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Features
■ Wide sample range: Suitable for depletion of rRNA in high quality (complete) and partially degraded (e.g. FFPE) samples.
■ Efficient removal: 95-99.9% of human/mouse/rat rRNA is effectively removed.
■ Comprehensive data: incomplete mRNA and non-coding RNA information are retained, making transcriptome data more comprehensive.
■ Rapid evaluation: The primers provided in the kit can quickly evaluate the removal effect of rRNA.
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Applications
This kit is suitable for the construction of RNA transcriptome library. It can also be used for random primer cDNA synthesis or other downstream applications.