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RNA Library Kits/Modules

TIANSeq rRNA Depletion Kit (H/M/R)

Rapidly and efficiently depletion of ribosomal RNA, which increase the proportion of effective sequencing data

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992391

 GNR101-03 96 rxn

4992364

 GNR101-02 24 rxn

4992363

 GNR101-01 6 rxn
Manual Inquire Inspection report

  • Storage

    This kit should be stored at-15~-25°C.

  • Description

    TIANSeq rRNA Depletion Kit (H/M/R) uses a specially designed DNA probe to hybridize with ribosomal RNA(rRNA), and then uses Ribo RNase H to degrade rRNA in the DNA-rRNA hybrid chain, thus removing cytoplasmic ribosomal RNA(28S, 18S, 5.8S, 5S) and mitochondrial ribosomal RNA(16S, 12S) in human, mouse and rat total RNA, and retaining messenger RNA(mRNA) and other non-coding RNA.

    The kit has a good rRNA removal effect on complete and partially degraded total RNA (such as FFPE RNA).The obtained rRNA-removed RNA sample can be used for mRNA and non-coding RNA high-throughput sequencing. The effective data ratio in sequencing results can be obviously improved, and the purified product can also be used for random primer cDNA synthesis or other downstream applications.

  • Required Reagents

    TIANSeq RNA Clean Beads (Cat#4992360/4992362/4992867)

    Ethanol

  • Features

    ■ Wide sample range: Suitable for depletion of rRNA in high quality (complete) and partially degraded (e.g. FFPE) samples.

    ■ Efficient removal: 95-99.9% of human/mouse/rat rRNA is effectively removed.

    ■ Comprehensive data: incomplete mRNA and non-coding RNA information are retained, making transcriptome data more comprehensive.

    ■ Rapid evaluation: The primers provided in the kit can quickly evaluate the removal effect of rRNA.

  • Applications

    This kit is suitable for the construction of RNA transcriptome library. It can also be used for random primer cDNA synthesis or other downstream applications.

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